2013, 34(6): 59-67.
Keywords： Scapharca broughtonii , Microsatellite , Geographic populations , Genetic diversity
利用多态性好的20对微卫星引物,对中国日照、黄岛、蓬莱和韩国的4个魁蚶地理群体进行了遗传多样性分析。结果显示,20个位点在4个群体中的等位基因数为3～17,平均等位基因数为8.35,平均有效等位基因数为6.230 6;观测杂合度(Ho)为0.466 7~0.966 7;期望杂合度(He)为0.619 8~0.931 8;多态信息含量(PIC)为0.530 1~0.909 3,表现出高的遗传多样性水平。4个群体间的遗传分化指数(Fst)在0.013 2~0.031 4之间,呈现出较低的遗传分化。群体间的遗传距离在0.125 5~0.245 8之间;通过构建UPGMA聚类树,显示日照群体和黄岛群体最先聚类,再与蓬莱群体聚类,最后与韩国群体聚类,说明中国群体与韩国群体亲缘关系较远。
Genetic diversity in four geographic populations of Scapharca broughtonii from Rizhao, Huangdao, Penglai, and Korea was analyzed using twenty SSR with high polymorphism obtained in our laboratory. Results showed that the number of alleles obtained from different populations ranged in 3-17.The average number of alleles and effective alleles were 8.35 and 6.230 6, respectively. The observed heterozygosity(Ho) and the expected heterozygosity(He) ranged in 0.466 7-0.966 7 and 0.619 8-0.931 8, respectively.The polymorphism information content(PIC) ranged in 0.530 1-0.909 3, indicating a high level of genetic diversity in these populations.The genetic differentiation index (Fst) of the four geographic populations ranged in 0.013 2-0.031 4, revealing a low level of genetic differentiation among them. The genetic distances among populations ranged in 0.125 5-0.245 8. The UPGMA tree indicated that Rizhao and Huangdao populations clustered first,then clustered with Penglai population, and further clustered with the Korean population, indicating that the Korean population was the farthest relative of the Chinese populations. The information of population structure obtained in this work would be helpful for the genetic breeding and conservation of S. broughtonii.
| [1] | |
| [2] | |
| [3] | |
| [4] | |
| [5] | |
| [6] | |
| [7] | |
| [8] | |
| [9] | |
| [10] | |
| [11] | |
| [12] | |
| [13] | |
| [14] | |
| [15] | |
| [16] | |
| [17] | |
| [18] | |
| [19] | |
| [20] | |
| [21] | |
| [22] | |
| [23] | |
| [24] | |
| [25] |
| [1] | 陈晶, 聂青, 刘妍. 《WHO基本药物示范目录》与我国《国家基本药物目录》动态调整程序比较与借鉴.水产学报,2015(3): 289-293.doi:10.3866/PKU.WHXB201503022 |