首页 >  海洋渔业 >  药浴暴露下阿维菌素在异育银鲫体内蓄积和消除规律

2014, 36(5): 461-468.

药浴暴露下阿维菌素在异育银鲫体内蓄积和消除规律

1. 中国水产科学研究院东海水产研究所, 农业部东海与远洋渔业资源开发利用重点实验室, 上海 200090;

2. 上海海洋大学水产与生命学院, 上海 201306;

3. 宁波市检验检疫科学技术研究院, 浙江宁波 315012

通讯作者: 房文红, fwenhong@163.com

收稿日期:2014-05-19

基金项目:   公益性行业(农业)科研专项(201203085) 

关键词: 阿维菌素 , 异育银鲫 , 蓄积 , 消除规律 , 药浴 , 休药期

Accumulation and elimination of Avermectin in Carassius auratus gibelio following medicated bath exposure

1. Key Laboratory of East China Sea and Oceanic Fishery Resources Exploitation and utilization, Ministry of Agriculture, East China Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Shanghai 200090, China;

2. College of Fisheries and Life Science, Shanghai Ocean University, Shanghai 201306, China;

3. Ningbo Academy of Science and Technology for Inspection and Quarantine, Ningbo, Zhejiang 315012, China

Corresponding author: FANG Wen-hong, fwenhong@163.com

Received Date:2014-05-19

Keywords: Avermectin , Carassius auratus gibelio , accumulation , elimination , bath exposure , withdrawal period

采用UPLC-MS/MS法研究了2μg·L-1三次连续水体药浴和6μg·L-1一次性水体药浴条件下阿维菌素在水体中消除、在异育银鲫(Carassius auratus gibelio)体内蓄积和消除变化规律。结果显示,两种药浴暴露方式下阿维菌素在水体中消除呈一级指数衰退消除,消除半衰期(t1/2)均为63 h,240 h时浓度下降到0.5μg·L-1以下。阿维菌素在异育银鲫血浆和肌肉中的含量均呈先升高后下降的趋势,血浆中药物浓度远高于肌肉中的含量。2 μg·L-1连续三次药浴组和6 μg·L-1一次药浴组血药峰浓度(Cmax)分别为34.97、66.62 μg·L-1,其曲线下面积(AUC0-t)分别为9 871.2 μg·L-1·h和18 119.6 μg·L-1·h;两组药浴肌肉中达峰浓度分别4.42 μg·kg-1和15.80 μg·kg-1,其AUC0-t分别为641.9 μg·kg-1·h和4 271.0μg·kg-1·h。与2 μg·L-1连续三次药浴组相比,6 μg·L-1一次药浴组阿维菌素在血浆和肌肉中的蓄积作用更加显著。以10 μg·kg-1作为阿维菌素在异育银鲫肌肉中最大残留限量,选择95%的置信区间计算异育银鲫肌肉组织中休药期,本研究中2 μg·L-1连续三次药浴组肌肉的休药期为295.4 h,6 μg·L-1一次药浴组肌肉的休药期为454.5 h。

In this paper,ultra high performance liquid chromatography tandem mass spectrometer (UPLCMS/MS) was used to detect Avermectin in water and biological samples based on the optimized pretreatment.Using this detection method,the elimination of Avermectin in water,the accumulation and elimination of Avermectin in Carassius auratus gibelio were studied following bath exposure at three consecutive doses of 2 μg·L-1 every other day and at a single dose of 6 μg·L-1 to investigate the impact on aquatic animals after bath exposure and provide basic data for safety evaluation of Avermectin in aquaculture.The limits of detection (LOD) in water,plasma and muscle were 0.03 μg·L-1,0.15 μg·kg-1 and 0.2 μg·kg-1,respectively,and the limits of quantitation (LOQ) were 0.10 μg·L-1,0.4 μg·L-1,0.5 μg·kg-1,respectively.The linear range in water,plasma and muscle were 0.1-50 μg·L-1,0.4-75 μg·L-1and 0.5-50 μg·kg-1,respectively,which showed good linearity,and the average recoveries were between 85%-115%.The detection method could meet the normal residual analysis requirements.Results of bath exposure showed that the Avermectin in water was eliminated by first-order exponential rate and half-life times (t1/2) were 63 h for both three consecutive exposure and the single exposure.The Avermectin concentrations in water were below 0.5 μg·L-1 in 240 h.The Avermectin concentrations in plasma and muscle increased at first following bath exposure and then decreased after peak time.The avermectin concentrations in plasma were significantly higher than that in muscle.The peak concentration (Cmax) of plasma following three consecutive dosage and a single dosage were 34.97 μg·L-1 and 66.62 μg·L-1,respectively;while the area under the concentration-time curve (AUC0-t) were 9 871.2 μg·L-1·h and 18 119.6 μg·L-1·h,respectively.The Cmaxin muscle were 4.42 μg·kg-1and 15.80 μg·kg-1,respectively,and AUC0-t were 641.9 μg·kg-1·h and 4 271.0 μg·kg-1·h,respectively.Accumulation of Avermectin in blood and muscle after a single exposure was more significant than that following three consecutive exposure.Regarding 10 μg·kg-1 as the maximum residue limit (MRL) of Avermectin in muscle,the withdrawal period of Avermectin in muscle was 295.4 h and 454.5 h for three consecutiveexposure of 2 μg·L-1 and single exposure of 6 μg·L-1 by the 95% confidence interval analysis.These results provided guiding significance for fishery drugs usage and establishment of withdrawal period in aquaculture.

参考文献

[1] ARENA J P,LIU K K,PARESS P S,et al.Expression of a glutamate-activatedchloride current in Xenopus ooeyted injected with Caenorhabditiselegans RNA:evidence for modulation by avermectin[J]. Molecular Brain Research,1992,15(3-4):339-387.
[2] 周帅,房文红,吴淑勤.渔用阿维菌素水乳剂的安全性和药效评价[J].上海海洋大学学报, 2009,18(3):327-331.
[3] 钟诗群.大水体鲢鳙鱼中华鳋病的防治与预防[J].科学养鱼,2013(1):64-65.
[4] 何晓明,关亚鹏,徐亲民.生物农药阿维菌素的发展[J].河北化工,2007,30(9):9-11.
[5] ELBETIEHA A,DA'AS S I.Assessment of antifertility activities of abamectin pesticide in male rats[J]. Eeotoxieology and Environmental Safety,2003,55(3):307-313.
[6] CHUNG Kong,YANG Chen-chang,wu Ming.1ing,et al.Agricultural avermeetins:an uncommon but potentially fatal cause of pesticide poisoning[J]. Annals of Emergency Medicine,1999,34(1):5l-57.
[7] CELIK-OZENCIL C,TASATARGIL A,TEKCAN M,et al.E[feet of abamectin exlxIsure on semen parametersindicative of reduced sperm maturity:a study on farmworkers in Antalya(Turkey)[J]. Andrologia,2012,44(6):388-395.
[8] (EC)No 868/2002 Commission Regulation[S].
[9] (EC)No 508/1999 Commission Regulation[S].
[10] 中华人民共和国农业部第235号公告动物源性食品中兽药最高残留限量[S].北京,2020.
[11] 王锡珍.阿维菌素对几种淡水生物毒性及在异育银鲫体内药物代谢动力学[D].上海:上海海洋大学,2009.
[12] 周海明,姚芳.高效液相色谱-荧光(HPLC-FD)检测定土壤和水中阿维菌素及其代谢产物残留量研究[J].安徽农业科学,201l,39(26):16037-16038,16096.
[13] 阮记明,胡鲲,杨先乐,等.异育银鲫体内阿维菌素血脑屏障渗透性及组织残留[J].中国水产科学,2013,20(5):103l-1038.
[14] 谢显传,张少华,王冬生,等.阿维菌素荧光衍生反应影响因子的研究[J].分析化学,2006,34(12):1745-1748.
[15] MONTIGNY P,SHIM J S K,PIVNICHNY J V,et al. Liquid chromatographic determination of ivermectin in animal plasma with trifluoroacetic anhydride and N-methylimidazole as the derivatization reagent[J]. Journal of Pharmaceutical and Biomedical Analysis, 1990,8(6):507-511.
[16] 高华鹏,张健玲,李永夫,等.超高效液相色谱-串联质谱法快速测定鳗鱼中阿维菌素类农药残留[J].质谱学报,2008,29(6):337-342.
[17] REZAEEM,MASHAYEKHIH A,SALEHA,et al. Determination of abamectin in citrus fruitsusing SPE combined with dispersive liquid-liquid microextraction and HPLC-UV detection[J].J. Sep.Sci.,2013,36(16):2629-2634.
[18] RUBENSAMG,BARRETOF,HOFFR B.et al. Determination of avermectin and milbemycin residues in bovine muscle by liquid chromatography-tandem mass spectrometry and fluorescence detection using solvent extraction and low temperature cleanup [J]. Food Control.,2013,29(1):55-60.
[19] 邢丽红,孙伟红,冷凯良,等.阿维菌素在鲈鱼组织中的富集及消除规律研究[J].中国渔业质量与标准,2013(4):63-69.
[20] 张启迪.阿维菌素在鲟鱼体内生物富集与生物消除规律的研究[D].北京:中国农业大学.2003.
[21] 秦改晓,徐文彦,艾晓辉,等.阿维菌素在草鱼体内的药物代谢动力学研究[J].西北农林科技大学学报(自然科学版),2012,40(8):13-20.
[22] 陈静.鲫鱼肌肉中阿维菌素类残留检测和消除规律研究[D].重庆:西南大学,2008.
[23] WILLIAM C C.Ivermectin and Abamectin[M].New York:Springer-Verlag.1989:73-88.
[24] 王广基.药物代谢动力学[M].北京:化学工业出版社,2005:68-70.

相关文章

[1] 陈晶, 聂青, 刘妍. 《WHO基本药物示范目录》与我国《国家基本药物目录》动态调整程序比较与借鉴.水产学报,2015(3): 289-293.doi:10.3866/PKU.WHXB201503022
  • 导出引用
  • 下载XML
  • 收藏文章
计量
  • 文章下载量()
  • 文章访问量()

目录

药浴暴露下阿维菌素在异育银鲫体内蓄积和消除规律