ETIOLOGICAL AND HISTOPATHOLOGICAL STUDY ON HEPATOPANCREATIC NECROSIS SYNDROME IN ERIOCHEIR SINENSIS
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摘要: 为了探讨中华绒螯蟹“水瘪子”病病因, 对病蟹进行了寄生虫检查、病原生物分离、回接攻毒及电镜观察等病原学研究, 同时采用常规石蜡切片技术, 以健康蟹为对照, 对病蟹的不同组织进行了病理学观察。结果表明, 病原分析未见致病性生物; 病蟹的鳃、肌肉和肝胰腺发生了不同程度的病变, 主要的病理特征为鳃组织增厚, 鳃腔增大, 血细胞增多, 细胞核边缘化; 肝胰腺组织中单层上皮细胞空泡化, 并出现转运泡, 随着病情的加重, 肝细胞排列紊乱, 转运泡和空泡的数量增多, 体积增大, 且转运泡内内容物增多, 更甚者肝小管基膜破裂、内容物外流, 细胞核解体, 肝细胞出现坏死; 肌细胞的病变特征主要是肌丝松弛变性、细胞核固缩深染。病原学研究和发病情况调查表明该病为非生物性疾病, 依据该病的临床症状、病理变化以及发病原因, 将该病命名为中华绒螯蟹肝胰腺坏死综合症。Abstract: A disease called " Shuibiezi” in Eriocheir sinensis was prevailed on Xinghua city, Jiangsu province since 2015, and slowly spread to other main areas of crab rearing in the whole nation. To explore the causes, the etiology by parasites examination, pathogenic bacteria isolation, challenge experiment and electron microscopy observation, and the histopathology by routine paraffin-section technique were conducted with healthy E. sinensis as control groups. Results showed that no pathogenic microorganism was detected by etiology research. Pathological changes with different degrees were observed in gills, muscles and hepatopancreas of diseased crabs, and the main pathological characteristics were gill tissue thickening, enlarged gill cavity, increased number of hemocyte, and marginalized nucleus. Vacuolization of the epithelial cells of the hepatopancreas and transferred vacuoles appeared. With the severity of the conditions, hepatic cells were irregular-arranged, and the number and volume of vacuoles and transferred vacuoles increased, and the number of granular materials in transferred vacuoles increased. With the most severe disease, the hepatic tubles were so ruptured that substances in cells were leaked, and the nucleus of hepatic cells were broken and hepatic cells appeared necrosis. The main lesion characteristics of muscle were muscular fibers appeared to be relaxed and pyknotic of nuclei. Etiology and incidence investigation revealed that the disease was caused by non living agents. Based on the main pathological characteristics and pathogenesis, this disease nominate scientifically as hepatopancreatic necrosis syndrome.
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Key words:
- Eriocheir sinensis /
- Hepatopancreatic necrosis syndrome /
- Etiology /
- Histopathology
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图 图版Ⅰ 对照组和患病中华绒螯蟹肝胰腺组织病理观察
Figure 图版Ⅰ. The histology of hepatopancreas of control group and disease Chinese mitten crab
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