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2019, 9(4): 56-63. doi: 10.3969/j.issn.2095-1833.2019.04.008

基于UHPLC-ESI-HRMS的仿刺参磷脂轮廓产地差异比较

1. 中国海洋大学食品科学与工程学院, 青岛 266003;

2. 青岛海关检验检疫技术中心, 青岛 266000

收稿日期:2019-03-22
修回日期:2019-05-10

基金项目:   国家自然科学基金(31330060);原国家质检总局科技计划项目(2016IK204) 

关键词: 仿刺参 , 超高效液相色谱-四极杆飞行时间串联质谱 , 产地溯源 , 磷脂轮廓 , 主成分分析 , 正交偏最小二乘法-判别分析(OPLS-DA)模型 , 磷脂标志物

Analysis of the phospholipid profiles and origin traceability of A. japonicus based on UHPLC-ESI-HRMS method

1. College of Food Science and Engineering, Ocean University of China, Qingdao 266003, China;

2. Technical Center of Shandong Inspection and Quarantine, Qingdao Customs, Qingdao 266000, China

Received Date:2019-03-22
Accepted Date:2019-05-10

Keywords: A. japonicus , UHPLC-ESI-TOF-HRMS , origin traceability , phospholipid profiles , PCA , OPLS-DA , phospholipid marker

对不同产地仿刺参(Apostichopus Japonicus)磷脂轮廓进行分析,并筛选潜在的产地差异磷脂标志物,为产地溯源提供方法学参考。通过超高效液相色谱-四极杆飞行时间串联质谱(UHPLC-ESI-TOF-HRMS)对不同产地(胶南、威海和大连)仿刺参磷脂轮廓进行分析,采用主成分分析法(PCA)对不同产地的仿刺参进行聚类分析,并构建正交偏最小二乘法-判别分析(OPLS-DA)模型,用于寻找不同产地仿刺参的磷脂分子标志物。结果表明,在仿刺参样品中共鉴定出160个磷脂分子种,据此构建的OPLS-DA模型[R2X=0.889(cum),R2Y=0.979(cum),Q2=0.954(cum)]能够实现对3种不同产地仿刺参的有效区分和聚类,并筛选出以磷脂酰乙醇胺(PE)和磷脂酰胆碱(PC)为主的10个产地差异磷脂分子标志物。本研究所构建的方法能够基于磷脂轮廓对不同产地仿刺参进行有效区分,可应用于仿刺参的产地鉴别和溯源工作,为高值海产品的溯源研究提供方法学参考,并为评价不同产地仿刺参营养价值差异研究提供数据支持。

This study focused on analyzing the difference between phospholipid profiles in A. japonicus from different geographical origins and finding the potential phospholipid biomarkers for origin traceability of A. japonicus. Firstly, UHPLC-ESI-TOF-HRMS was used to analyze the A. japonicus from different geographical origins (Jiaonan, Weihai and Dalian), and then principal component analysis (PCA) was used to cluster phospholipid profiles of A. japonicus from different geographical origins. An orthogonal partial least squares-discriminant analysis (OPLS-DA) model was established to find phospholipid molecular biomarkers of A. japonicus from different geographical origins. A total of 160 phospholipid molecular species were identified in A. japonicus. The effective differentiation and clustering of A. japonicus from different geographical origins were achieved by OPLS-DA model[R2X=0.889 (cum), R2Y=0.979 (cum), Q2=0.954 (cum)]. There were 10 phospholipid biomarkers found in A. japonicus from three geographical origins, and most of them were PE and PC. The method in this paper could effectively distinguish A. japonicus from different geographical origins, which had potential to be applied in the origin traceability of high-value seafood such as A. japonicus. In addition, it also provided data support for evaluating the nutritional value of A. japonicus from different geographical origins.

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基于UHPLC-ESI-HRMS的仿刺参磷脂轮廓产地差异比较