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ISSN 2095-1833

主管 中华人民共和国农业部

主办 中国水产科学研究院

水产品中孔雀石绿BA-ELISA检测方法的建立及应用

杨光昕 苏悦 李冰莲 孔聪 黄宣运 张璇 于慧娟 韩峰

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杨光昕, 苏悦, 李冰莲, 孔聪, 黄宣运, 张璇, 于慧娟, 韩峰. 2019. 水产品中孔雀石绿BA-ELISA检测方法的建立及应用. 中国渔业质量与标准, 9(4): 30-35. doi: 10.3969/j.issn.2095-1833.2019.04.005
引用本文: 杨光昕, 苏悦, 李冰莲, 孔聪, 黄宣运, 张璇, 于慧娟, 韩峰. 2019. 水产品中孔雀石绿BA-ELISA检测方法的建立及应用. 中国渔业质量与标准, 9(4): 30-35. doi: 10.3969/j.issn.2095-1833.2019.04.005
YANG Guangxin, SU Yue, LI Binglian, KONG Cong, HUANG Xuanyun, ZHANG Xuan, YU Huijuan, HAN Feng. 2019. Establishment and application of BA-ELISA for malachite green detection in aquatic products. Chinese Fishery Quality and Standards, 9(4): 30-35. doi: 10.3969/j.issn.2095-1833.2019.04.005
Citation: YANG Guangxin, SU Yue, LI Binglian, KONG Cong, HUANG Xuanyun, ZHANG Xuan, YU Huijuan, HAN Feng. 2019. Establishment and application of BA-ELISA for malachite green detection in aquatic products. Chinese Fishery Quality and Standards, 9(4): 30-35. doi: 10.3969/j.issn.2095-1833.2019.04.005

水产品中孔雀石绿BA-ELISA检测方法的建立及应用

  • 基金项目:

    中央级公益性科研院所基本科研业务费专项(2016T09)

详细信息
    作者简介:

    杨光昕(1984-),男,博士,助理研究员,研究方向为水产品质量安全,yang_sh33@163.com

  • 中图分类号: S91

Establishment and application of BA-ELISA for malachite green detection in aquatic products

  • Fund Project: 中央级公益性科研院所基本科研业务费专项(2016T09)
  • 建立了基于生物素-链霉亲合素放大的酶联免疫吸附法(BA-ELISA)检测水产品中的孔雀石绿(MG)含量的方法。优化了包被原和生物素化抗体的工作浓度,以及封闭液、温育时间、pH值和盐离子强度等实验条件。结果显示,最佳实验条件为:包被原和生物素化抗体的稀释倍数均为1∶2 000,封闭液为1%明胶,抗原抗体反应时间为60 min,缓冲液的pH值为7.0,盐离子强度为0.1 mol/L。在此条件下,该方法的线性范围为0.05~20.00 ng/mL,检出限(LOD)为0.037 μg/kg,回收率为83.5%~115.0%,变异系数(CV)为4.53%~13.80%。将该方法用于实际样品的分析检测,检测结果与LC/MS相比,具有较好的相关性。上述结果表明,本实验建立的方法灵敏度高,特异性和稳定性较好,可用于水产品中MG的分析筛查。
  • 加载中
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  • 文章访问数:  2519
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  • 施引文献:  0
出版历程
收稿日期:  2019-04-01
修回日期:  2019-06-24

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