Anesthetic Effect of Eugenol on Sebastiscus Marmoratus
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摘要: 【目的】探究丁香酚对标志规格褐菖鲉（Sebastiscus marmoratus）［体长（88.12±4.87）mm］的麻醉效果，为褐菖鲉标志放流技术提供参考依据。【方法】设置10～180 mg/L共12个丁香酚质量浓度组进行实验，观察不同浓度下鱼体可达到的最终麻醉阶段，初步筛选出适宜的浓度范围，依据麻醉复苏时间以及复苏率进一步确定有效麻醉质量浓度，在此基础上进行空气暴露实验比较研究。【结果】褐菖鲉在大于30 mg/L质量浓度中可在3 min内达到Ⅳ期麻醉，在小于80 mg/L质量浓度中麻醉5 min未出现死亡及明显应激反应，初步筛选得到适宜浓度范围为30～80 mg/L；参考常用有效麻醉质量浓度选定标准，结合复苏实验得到褐菖鲉有效麻醉浓度为40～50 mg/L，此时褐菖鲉可在3 min内入麻、7 min内复苏、麻醉15 min后成活率100%；褐菖鲉在小于150 mg/L溶液中麻醉5 min全部存活，麻醉5 min以上时多组出现死亡；在40 mg/L浓度下进行空气暴露实验，15 min时平均复苏时间最短，之后平均复苏时间随空气暴露时间的延长逐步增加，由此认为在空气中15 min时褐菖鲉已复苏，为尽量避免鱼体应激，应在15 min内完成离水实验操作。【结论】丁香酚对标志规格褐菖鲉的适宜麻醉浓度为40～50 mg/L，离水操作应控制在15 min内，若需快速麻醉可选用50～150 mg/L丁香酚，并控制麻醉时间在5 min内以免造成麻醉致死。Abstract: 【Objective】 To explore the anesthetic effect of eugenol on Sebastiscus marmoratus, and to provide reference for the tagged releasing technology of S. marmoratus.【Method】 A total of 12 eugenol concentration groups of 10-180 mg/L were set up to observe the final anesthesia stage that the fish could reach at different concentrations, and the appropriate concentration range was preliminarily screened.The effective anesthesia concentration was further determined according to the anesthesia recovery time and recovery rate.On this basis, the comparative study of air exposure test was carried out.【Result】 S. marmoratus could reach stage IV anesthesia within 3 min at a concentration of more than 30 mg/L, and no death and obvious stress response occurred within 5 min at a concentration of less than 80 mg/L.The suitable concentration range was 30 -80 mg/L after preliminary screening.According to the commonly used effective anesthesia mass concentration selection criteria, combined with the resuscitation test, the effective anesthesia concentration of S. marmoratus was 40 -50 mg/L.At this time, S. marmoratus could be anesthetized within 3 min, resuscitated within 7 min, and the survival rate was 100% after 15 min of anesthesia.All the S. marmoratus survived in the solution less than 150 mg/L for 5 min, and died in more than 5 min; In the air exposure test at a concentration of 40 mg/L, the average recovery time was the shortest at 15 min, and then the average recovery time gradually increased with the extension of air exposure time.Therefore, it is considered that S. marmoratus has recovered at 15 min in the air.In order to avoid the stress of the fish, the water-leaving test operation should be completed within 15 min.【Conclusion】 The suitable anesthetic concentration of eugenol for the marker specification of S. marmoratus [body length (88.12 ± 4.87) mm)] is 40 -50 mg/L, and the operation of leaving water should be controlled within 15 min.If rapid anesthesia is required, 50 -150 mg/L can be selected, and the anesthesia time should be controlled within 5 min to avoid anesthesia death.
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Key words:
- Sebastiscus marmoratus /
- eugenol /
- anesthesia and resuscitation /
- anesthetic effect
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