Effects of DHA-enriched Phosphatidylcholine on Bisphenol S-induced Liver Injury in Mice
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摘要: 【目的】研究从大西洋鲱（Clupea harengus）鱼籽中提取的富二十二碳六烯酸-磷脂酰胆碱（DHA-PC）对双酚S（BPS）诱导的小鼠肝损伤的改善作用。【方法】将24只雄性C57BL/6小鼠随机分成对照组、BPS模型组和DHA-PC给药组，每组8只，模型组和给药组分别给予5 mg·（kg·d）-1 BPS，给药组同时灌胃80 mg·（kg·d）-1 DHAPC，饲养10周。监测各组小鼠的食物摄入量、体质量、肝功能、附睾脂肪指数、肝脏组织病理学分析、肝脏组织中炎症因子和氧化应激等指标，研究DHA-PC对BPS诱导的小鼠肝损伤的改善作用。【结果】BPS处理后，模型组小鼠体质量、肝脏指数、附睾脂肪指数显著升高（P＜0.05），但经DHA-PC饲喂治疗后，均呈现下降趋势。所有组别小鼠的食物摄入量、能量摄入量无差异（P＞0.05）。病理学结果显示模型组小鼠肝脏肝索消失，肝脏发生局灶性脂肪性病变，给药组肝索恢复，中央静脉重现。与模型组相比，DHA-PC给药组显著降低谷丙转氨酶（ALT）、谷草转氨酶（AST）、白介素（IL）-1β、IL-6、肿瘤坏死因子（TNF）-α、胆固醇（TC）、甘油三酯（TG）、游离脂肪酸（NEFA）、低密度脂蛋白（LDL-C）和丙二醛（MDA）的含量（P＜0.05），显著提高高密度脂蛋白（HDL-C）、总抗氧化能力（T-AOC）、超氧化物歧化酶（SOD）、谷胱甘肽过氧化物酶（GSH-Px）和过氧化氢酶（CAT）的含量（P＜0.05）。【结论】鲱鱼鱼籽源DHA-PC可通过抑制炎症、改善脂质代谢紊乱和氧化应激等途径有效改善BPS诱导的小鼠肝损伤。
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关键词:
- 二十二碳六烯酸-磷脂酰胆碱 /
- 双酚S /
- 小鼠 /
- 肝损伤
Abstract: 【Objective】 The aim of this study is to study the ameliorative effect of docosahexaenoic acid-enriched phosphatidylcholine (DHA-PC) on bisphenol S (BPS) -induced liver injury in mice.【Methods】 C57BL/6 mice (n=24) were randomly divided into control group, model group and administration group with 8 mice in each group.The model group and administration group were given 5 mg·(kg·d)-1 BPS for 10 weeks, respectively, at the same time, the administration group was given 80 mg/kg DHA-PC.The protective effect of DHA-PC [80 mg·(kg·d)-1]on BPS-induced liver injury in mice was studied by monitoring the food intake and body weight of mice, liver function and liver histopathological analysis, inflammatory factors, lipids and oxidative stress in liver tissue.【Results】 After BPS treatment, the body mass, liver index and epididymal fat index of mice were significantly increased (P＜0.05), and after DHA-PC treatment, these indices showed a downward trend.There was no difference in food intake or energy intake among the three groups (P＞0.05).Pathological results showed that the hepatic cord disappeared and focal fatty lesion occurred in the model group, while the hepatic cord recovered and central vein reappeared in the administration group.Compared with the model group, the contents of alanine aminotransferase (ALT), aspartate aminotransferase (AST), interleukin (IL)-1β, IL-6, tumor necrosis factor (TNF)-α, cholesterol (TC), triglyceride (TG), free fatty acid (NEFA), low-density lipoprotein cholesterol (LDL-C) and malondialdehyde (MDA) were significantly decreased after DHA-PC treatment (P＜0.05).The contents of high-density lipoprotein cholesterol (HDL-C), total antioxidant capacity (T-AOC), superoxide dismutase (SOD), glutathione peroxidase (GSH-Px) and catalase (CAT) were significantly increased after DHA-PC treatment, compared with the model group (P＜0.05).【Conclusion】 DHA-PC from herring roe can effectively improve BPS-induced liver injury in mice by inhibiting inflammation, improving lipid metabolism disorder and oxidative stress.-
Key words:
- docosahexaenoic acid-enriched phosphatidylcholine /
- bisphenol S /
- mice /
- liver injury
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