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ISSN 1673-9159

主管 广东省高等教育厅

主办 广东海洋大学


2023. CO2麻醉对大口黑鲈保活模拟运输效果的影响. 广东海洋大学学报, 43(6): 62-71. doi: 10.3969/j.issn.1673-9159.2023.06.008
引用本文: 2023. CO2麻醉对大口黑鲈保活模拟运输效果的影响. 广东海洋大学学报, 43(6): 62-71. doi: 10.3969/j.issn.1673-9159.2023.06.008
WANG Zhaoding, LI Ning, BAI Chan, WANG Juguang, CHAI Yi, XIONG Guangquan. 2023. Effects of CO2 Anaesthesia on Keeping Alive Largemouth Bass in the Simulated Transport. Journal of Guandong Ocean University, 43(6): 62-71. doi: 10.3969/j.issn.1673-9159.2023.06.008
Citation: WANG Zhaoding, LI Ning, BAI Chan, WANG Juguang, CHAI Yi, XIONG Guangquan. 2023. Effects of CO2 Anaesthesia on Keeping Alive Largemouth Bass in the Simulated Transport. Journal of Guandong Ocean University, 43(6): 62-71. doi: 10.3969/j.issn.1673-9159.2023.06.008


  • 基金项目:




  • 中图分类号: S981.1

Effects of CO2 Anaesthesia on Keeping Alive Largemouth Bass in the Simulated Transport

  • Fund Project: 财政部和农业农村部国家现代农业产业技术体系专项(CARS-46);湖北省科技重大项目(2023BBA001);湖北省农业科技创新中心重大科技研发专项(2020-620-000-002-03);湖北省重点研发计划项目(2023BBB122)
  • 【目的】探讨CO2麻醉辅助大口黑鲈(Micropterus salmoides)保活模拟运输时的温度、CO2浓度和运输时间的选择,评价大口黑鲈模拟运输过程中血清生化指标和肌肉营养成分的变化。【方法】从暂养水箱中随机取20尾大口黑鲈放置在装有15 L曝气后的实验用水的玻璃鱼缸中,采用单因素实验,以存活率为指标,确定最佳运输温度和CO2浓度;在最佳水温和CO2质量浓度下进行保活模拟运输,分别在保活模拟运输的0、12、24、36和48 h取水样和血样,测定水质、血清生化指标、肌肉营养成分等指标。【结果】使用CO2麻醉大口黑鲈,在7℃下需要15.23 min,在14℃下需要10.33 min。麻醉后在7℃水温中复苏时间为10.20 min,14℃时为3.53 min。经过单因素实验,确定最佳运输温度为15℃,最佳CO2麻醉浓度为4.8、6.8 g/L,此条件下相较于只通入O2的保活模拟运输,大口黑鲈存活率更好,肌肉营养更佳,且在模拟运输36 h时存活率为100%。模拟运输36 h时,血清中皮质醇、葡萄糖含量,乳酸脱氢酶活性均显著高于运输前(P<0.05);谷草转氨酶、谷丙转氨酶活性,尿素氮、肌酐含量均显著低于运输前(P<0.05);肌肉中乳酸含量显著高于运输前(P<0.05),糖原和蛋白质含量均显著低于运输前(P<0.05)。对照组血清中谷草转氨酶、谷丙转氨酶活性,尿素氮、肌酐含量显著高于CO2处理组(P<0.05),说明CO2麻醉可以减轻运输中应激造成的肝肾损伤。【结论】在运输温度为15℃,6.8 g/L CO2条件下麻醉大口黑鲈进行保活模拟运输,应控制运输时间在36 h内。
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收稿日期:  2023-08-16