Effects of Compound Additive on Biochemical Parameters and Tissue Structure of Simulated Transport of Juvenile Ictalurus punctatus
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摘要: 【目的】探究复配水溶性添加剂（牛磺酸、维生素C、维生素E、谷氨酰胺和谷氨酸钠）对斑点叉尾鮰（Ictalurus punctatus）幼鱼运输过程中水质、生化参数以及组织结构的影响。【方法】向运输水中分别添加不同体积分数的复配水溶性添加剂（分别记为F-20%组、F-40%组、F-60%组、F-80%组、F-100%组），以不添加任何水溶性添加剂处理为对照组（Control），随后模拟实际运输过程14 h。考察模拟运输过程中（0、5、9、14 h）的水质（总氨氮、pH）、血清和肝脏生化参数及组织结构变化。【结果】运输14 h后，复配水溶性添加剂处理组水体总氨氮、血清皮质醇（COR）浓度和肝脏过氧化氢酶（CAT）活性、丙二醛（MDA）含量均显著低于对照组（P＜0.05），血清葡萄糖（GLU）含量除F-20%组外其他处理组均显著低于对照组（P＜0.05）；除F-100%组外，其他添加剂组的水体pH值显著高于对照组（P＜0.05）。运输14 h后，F-40%组的总抗氧化能力显著低于对照组和其他浓度实验组（P＜0.05）；鳃和皮肤结构损伤程度低于对照组。【结论】复配水溶性添加剂能够提高斑点叉尾鮰幼鱼有水运输过程中的抗应激能力，减少甚至免除换水操作，缩短运输时间且保持幼鱼的健康状态。Abstract: 【Objective】 The purpose of this study is to investigate the effects of a composite watersoluble additive(taurine, vitamin C, vitamin E, glutamine and sodium glutamate)on water quality, and on biochemical parameters and tissue structure of juvenile Ictalurus punctatus during transportation.【Methods】 Different mass concentrations of the composite water-soluble additive (named F-20%, F-40%, F-60%, F-80%, F-100% groups) were added to the water, with no additive group as the control group.Subsequently, a 14-hour transportation simulation was conducted, and the water quality (total ammonia nitrogen, pH), serum and liver biochemical parameters as well as tissue structure changes during the transportation simulation (0, 5, 9, 14 h) were evaluated.【Results】 After 14 hours of transportation, the concentrations of total ammonia nitrogen in the water, cortisol in the serum, and catalase and malondialdehyde in the liver were significantly lower in the groups treated with the composite water-soluble additive compared to the control group(P＜0.05).The glucose content in the serum was significantly lower in all treatment groups except for group F-20% compared to the control group(P＜0.05).Except for group F-100%, the pH values of the water in the other treatment groups were significantly higher than those in the control group (P＜0.05).After 14 h of transportation, the total antioxidant capacity of group F-40% was significantly lower than that of the control group and other treatment groups (P＜0.05).The degree of damage to the gills and skin structure of group F-40% was lower than that in the control group.【Conclusion】 The composite water-soluble additive can enhance the stress resistance of juvenile I. punctatus during transportation, reduce or even eliminate water changing operations, shorten transportation time, and maintain the health status of the juvenile I. punctatus.
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