2023, 43(2): 36-42. doi: 10.3969/j.issn.1673-9159.2023.02.005
关键词： 红鳍笛鲷 , 色素细胞 , 组织切片 , 类胡萝卜素
Keywords： Lutjanus erythropterus , pigment cells , tissue section , carotenoids
【目的】揭示红鳍笛鲷（Lutjanus erythropterus）红体色的分布特征及影响机制。【方法】利用色差仪分别测定背部、腹部和尾部皮肤的体色值，用形态学观察、石蜡切片、冰冻切片和透射电镜切片等方法分析色素细胞组成、分布和数量特征，并使用紫外分光光度法和液相色谱-质谱/质谱法研究类胡萝卜素和体色特征的关系。【结果与结论】红鳍笛鲷体表的红色素细胞显著多于黑色素细胞（P < 0.001），且从背部向腹部逐渐减少，红体色受到黑色素细胞分布的影响；皮肤和视网膜是类胡萝卜素的主要沉积部位，皮肤中类胡萝卜素以脂肪酸结合的形式存在，其中红色类胡萝卜素主要有虾青素、角黄素和β-柠乌素等3种，虾青素和角黄素含量与红色素细胞数量呈正相关。建议在饲料中补充虾青素和角黄素以提升红色。
【目的】揭示红鳍笛鲷（Lutjanus erythropterus）红体色的分布特征及影响机制。【方法】利用色差仪分别测定背部、腹部和尾部皮肤的体色值，用形态学观察、石蜡切片、冰冻切片和透射电镜切片等方法分析色素细胞组成、分布和数量特征，并使用紫外分光光度法和液相色谱-质谱/质谱法研究类胡萝卜素和体色特征的关系。【结果与结论】红鳍笛鲷体表的红色素细胞显著多于黑色素细胞（P < 0.001），且从背部向腹部逐渐减少，红体色受到黑色素细胞分布的影响；皮肤和视网膜是类胡萝卜素的主要沉积部位，皮肤中类胡萝卜素以脂肪酸结合的形式存在，其中红色类胡萝卜素主要有虾青素、角黄素和β-柠乌素等3种，虾青素和角黄素含量与红色素细胞数量呈正相关。建议在饲料中补充虾青素和角黄素以提升红色。
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