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ISSN 1673-9159

主管 广东省高等教育厅

主办 广东海洋大学

miRNA-155靶向SOCS5对无乳链球菌诱导罗非鱼脑星形胶质细胞炎症的影响

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2023. miRNA-155靶向SOCS5对无乳链球菌诱导罗非鱼脑星形胶质细胞炎症的影响. 广东海洋大学学报, 43(2): 1-10. doi: 10.3969/j.issn.1673-9159.2023.02.001
引用本文: 2023. miRNA-155靶向SOCS5对无乳链球菌诱导罗非鱼脑星形胶质细胞炎症的影响. 广东海洋大学学报, 43(2): 1-10. doi: 10.3969/j.issn.1673-9159.2023.02.001
ZHANG Xiang, HUANG Yu, CAI Jia, JIAN Ji-chang, WANG Bei. 2023. Effect of MiRNA-155-Targeted SOCS5 Gene on Inflammatory Response of Brain Astrocytes of Oreochromis niloticus Induced by Streptococcus agalactiae. Journal of Guandong Ocean University, 43(2): 1-10. doi: 10.3969/j.issn.1673-9159.2023.02.001
Citation: ZHANG Xiang, HUANG Yu, CAI Jia, JIAN Ji-chang, WANG Bei. 2023. Effect of MiRNA-155-Targeted SOCS5 Gene on Inflammatory Response of Brain Astrocytes of Oreochromis niloticus Induced by Streptococcus agalactiae. Journal of Guandong Ocean University, 43(2): 1-10. doi: 10.3969/j.issn.1673-9159.2023.02.001

miRNA-155靶向SOCS5对无乳链球菌诱导罗非鱼脑星形胶质细胞炎症的影响

  • 基金项目:

    国家自然科学基金项目 (32002426, U20A2065, 32073006)

详细信息
    作者简介:

    张祥,男,硕士研究生,研究方向为水生动物医学。E-mail:1647203730@qq.com

  • 中图分类号: S941.42

Effect of MiRNA-155-Targeted SOCS5 Gene on Inflammatory Response of Brain Astrocytes of Oreochromis niloticus Induced by Streptococcus agalactiae

  • Fund Project: 国家自然科学基金项目 (32002426, U20A2065, 32073006)
  • 【目的】探究尼罗罗非鱼(Oreochromis niloticus)微小RNA-155(miR-155)和SOCS5(Suppressor of cytokinesignaling 5)的靶向关系,并研究其对无乳链球菌诱导的尼罗罗非鱼脑星形胶质细胞炎症反应的影响。【方法】通过实时荧光定量PCR(qRT-PCR)检测感染无乳链球菌后尼罗罗非鱼脑星形胶质细胞中miR-155、SOCS5及炎症因子表达变化规律,生物信息学方法预测miR-155与SOCS5的靶向关系,双荧光素酶报告基因实验进行靶基因验证,qRT-PCR方法分析过表达和敲降miR-155对SOCS5基因表达的影响和miR-155对SOCS5的调控机制。【结果】无乳链球菌诱导尼罗罗非鱼脑星形胶质细胞中miR-155表达量极显著上升(P < 0.001),4 h达到最高值。SOCS5表达量极显著上升(P < 0.01),6 h达到最高值。促炎症因子IL-1βTNF-α表达显著升高(P < 0.000 1),抑炎症因子IL-10TGF-β表达显著下调(P < 0.05)。构建尼罗罗非鱼SOCS5 3′UTR野生型和突变型荧光素酶报告载体。双荧光素酶实验结果表明miR-155极显著抑制(P < 0.000 1) SOCS5野生型质粒表达。突变靶位点后,miR-155对SOCS5的抑制作用极显著降低(P < 0.000 1),抑制效果减弱53.7%。过表达miR-155能显著抑制(P < 0.001) SOCS5表达,而抑制miR-155后SOCS5表达显著上升(P < 0.05)。转染miR-155抑制物6 h和12 h后,miR-155表达量显著下调(P < 0.01),12 h表达量最低,而SOCS5表达量显著上调(P < 0.05),在12 h达到最高值。【结论】miR-155通过靶向负调控SOCS5的表达参与无乳链球菌诱导的尼罗罗非鱼脑星形胶质细胞炎症反应,通过下调SOCS5表达影响炎症变化。
  • 加载中
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出版历程
收稿日期:  2023-01-06

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