首页 >  广东海洋大学学报 >  盐度驯化对虹鳟幼鱼鳞片再生过程MMP2和MMP9基因表达的影响

2022, 42(5): 110-115. doi: 10.3969/j.issn.1673-9159.2022.05.014

盐度驯化对虹鳟幼鱼鳞片再生过程MMP2和MMP9基因表达的影响

1. 广东海洋大学水产学院, 广东 湛江 524025;

2. 南方海洋科学与工程广东省实验室(湛江), 广东 湛江 524025

收稿日期:2022-04-26
修回日期:2022-04-26

基金项目:   国家自然科学基金(31772828);广东海洋大学科研启动经费(R19022) 

关键词: 盐度驯化 , 再生鳞 , 虹鳟 , MMPs , 基因表达

Effects of Salinity Acclimation on Expression of MMP2 and MMP9 in Regenerated Scales of Young Rainbow Trout(Oncorhynchus mykiss)

1. College of Fisheries, Guangdong Ocean University, Zhanjiang 524025, China;

2. Southern Marine Science and Engineering Guangdong Laboratory(Zhanjiang), Zhanjiang 524025, China

Received Date:2022-04-26
Accepted Date:2022-04-26

Keywords: salinity acclimation , regenerated scales , Oncorhynchus mykiss , MMPs , gene expression

【目的】揭示MMP2 和MMP9 在虹鳟(Oncorhynchus mykiss)幼鱼鳞片再生过程中的作用及盐度驯化对鳞片再生的影响。【方法】基于虹鳟转录组数据提取MMP2 和MMP9 基因序列信息,基于二基因氨基酸序列进行MMPs基因的系统进化分析;设置盐度驯化组(盐度27)和对照组(盐度3),分别采集两组虹鳟幼鱼在鳞片再生过程中不同时间(1、3、7、14、21 d)的正常鳞和再生鳞样品,利用实时荧光定量PCR 技术检测MMP2 和MMP9 基因的表达水平。【结果】系统发育树分析表明,虹鳟MMP2 和MMP9 均与大西洋鲑(Salmo salar)相应MMPs 先聚为一支,再与其他鱼类MMPs聚为硬骨鱼类分支;对照组再生鳞MMP2和MMP9表达水平均总体呈先升后降趋势,在7 d时表达量最高;盐度驯化组再生鳞MMP2 基因表达水平变化趋势为先升后降,在3 d 时表达量达到最大值,而MMP9基因的表达水平呈降低趋势。【结论】MMP2 和MMP9 在鳞片再生过程中基因表达水平的变化可作为成骨细胞和破骨细胞增殖分化的特征之一;盐度驯化抑制了虹鳟鳞片再生过程中MMP2 和MMP9 表达水平,表明盐度变化对骨再生过程有抑制作用。

【Objective】In order to clarify the roles of MMP2 and MMP9 gene in the process of scale regeneration in young rainbow trout (Oncorhynchus mykiss) and the effect of salinity acclimation on scale regeneration.【Method】The cDNA sequences of MMP2 and MMP9 were identified based on previous transcriptome data, and the phylogenetic analysis were carried out based on deduced amino acid sequences.In addition, five sampling time points during scale regeneration (1, 3, 7, 14 and 21 d)were selected in both the salinity acclimation group (salinity 27) and the control group (salinity 3).The expression levels of MMP2 and MMP9 in both ontogenic scales and regenerated scales were detected using quantitative real-time PCR (qRT-PCR).【Result】The results of phylogenetic analysis showed that O.mykiss MMP2 and MMP9 both shared a high identity with those of Salmo salar MMPs, and teleost MMP proteins formed a single distinct lineage from other vertebrates.The expression levels of MMP2 and MMP9 in the regenerated scales of the control group fish showed an overall trend of firstly increase and then decrease, and the highest expression level was identified at 7 d.In the salinity acclimation group, the expression level of MMP2 firstly increased and then followed by a decrement, but the MMP9 mRNA level showed a decreasing trend.【Conclusion】The aforementioned results indicated that the changes in gene expression levels of MMP2 and MMP9 during scale regeneration could be one of the characteristics of proliferation and differentiation of osteoblast and osteoclast.Salinity acclimation inhibited the expression levels of MMP2 and MMP9 during scale regeneration of rainbow trout, indicating that the regeneration of bones could be inhibited by salinity change.

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盐度驯化对虹鳟幼鱼鳞片再生过程MMP2和MMP9基因表达的影响