首页 >  广东海洋大学学报 >  洗脱剂极性对沉积物长链烯酮硅胶柱层析效果的影响

2022, 42(5): 82-87. doi: 10.3969/j.issn.1673-9159.2022.05.010

洗脱剂极性对沉积物长链烯酮硅胶柱层析效果的影响

1. 广东海洋大学海洋与气象学院, 广东 湛江 524088;

2. 陆架及深远海气候、资源与环境广东省高等学校重点实验室, 广东 湛江 524088;

3. 广东海洋大学深圳研究院, 广东 深圳 518108

收稿日期:2022-04-17
修回日期:2022-04-17

基金项目:   国家重点研发计划课题(2018YFA0605601);国家自然科学基金(41606070);广东海洋大学科研启动经费资助项目(R17011) 

关键词: 长链烯酮 , 海洋沉积物 , 柱层析 , 洗脱剂 , 硅胶柱

Influence of Eluent Polarity on Separation of Sediment Long-Chain Alkenones in Silica Gel Column Chromatography

1. College of Ocean and Meteorology, Guangdong Ocean University, Zhanjiang 524088, China;

2. Key Laboratory of Climate, Resources and Environment in Continental Shelf Sea and Deep Sea of Department of Education of Guangdong Province, Zhanjiang 524088, China;

3. Shenzhen Institute of Guangdong Ocean University, Shenzhen 518108, China

Received Date:2022-04-17
Accepted Date:2022-04-17

Keywords: alkenones , marine sediment , column chromatography , elution solvent , silica gel column

摘要

【目的】研究洗脱溶剂极性对海洋沉积物中C37和C38烯酮的柱层析分离效果及对重建古海表温度的影响,探寻能完全洗脱长链烯酮而不引入过多杂质的洗脱溶剂配比。【方法】通过改变正己烷(H)与二氯甲烷(D)体积比来调节洗脱液极性,按极性从小到大对标准样品进行洗脱,并计算每种烯酮分子的洗脱量。【结果】当VHVD=8∶1时烯酮开始被洗脱出,到1∶2时可被完全洗脱。烯酮C38:2 Et最先被洗脱出,而C37:3最后被洗脱出。各烯酮分子被洗脱量的峰值出现在不同极性的洗脱液,按极性排序为C38:2 Et < C38:3 Et < (C38:2 Me、C37:2< C37:3。【结论】洗脱液极性对不同烯酮分子洗脱效果有差异,如果C37洗脱不完全,可能会使偏大,导致换算的温度偏高。VHVD小于1∶2时,烯酮分子可被完全洗脱。

【目的】研究洗脱溶剂极性对海洋沉积物中C37和C38烯酮的柱层析分离效果及对重建古海表温度的影响,探寻能完全洗脱长链烯酮而不引入过多杂质的洗脱溶剂配比。【方法】通过改变正己烷(H)与二氯甲烷(D)体积比来调节洗脱液极性,按极性从小到大对标准样品进行洗脱,并计算每种烯酮分子的洗脱量。【结果】当VHVD=8∶1时烯酮开始被洗脱出,到1∶2时可被完全洗脱。烯酮C38:2 Et最先被洗脱出,而C37:3最后被洗脱出。各烯酮分子被洗脱量的峰值出现在不同极性的洗脱液,按极性排序为C38:2 Et < C38:3 Et < (C38:2 Me、C37:2< C37:3。【结论】洗脱液极性对不同烯酮分子洗脱效果有差异,如果C37洗脱不完全,可能会使偏大,导致换算的温度偏高。VHVD小于1∶2时,烯酮分子可被完全洗脱。

参考文献

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洗脱剂极性对沉积物长链烯酮硅胶柱层析效果的影响