2022, 42(5): 82-87. doi: 10.3969/j.issn.1673-9159.2022.05.010
关键词： 长链烯酮 , 海洋沉积物 , 柱层析 , 洗脱剂 , 硅胶柱
Keywords： alkenones , marine sediment , column chromatography , elution solvent , silica gel column
【目的】研究洗脱溶剂极性对海洋沉积物中C37和C38烯酮的柱层析分离效果及对重建古海表温度的影响，探寻能完全洗脱长链烯酮而不引入过多杂质的洗脱溶剂配比。【方法】通过改变正己烷（H）与二氯甲烷（D）体积比来调节洗脱液极性，按极性从小到大对标准样品进行洗脱，并计算每种烯酮分子的洗脱量。【结果】当VH∶VD=8∶1时烯酮开始被洗脱出，到1∶2时可被完全洗脱。烯酮C38：2 Et最先被洗脱出，而C37：3最后被洗脱出。各烯酮分子被洗脱量的峰值出现在不同极性的洗脱液，按极性排序为C38：2 Et < C38：3 Et < （C38：2 Me、C37：2）< C37：3。【结论】洗脱液极性对不同烯酮分子洗脱效果有差异，如果C37洗脱不完全，可能会使偏大，导致换算的温度偏高。VH∶VD小于1∶2时，烯酮分子可被完全洗脱。
【目的】研究洗脱溶剂极性对海洋沉积物中C37和C38烯酮的柱层析分离效果及对重建古海表温度的影响，探寻能完全洗脱长链烯酮而不引入过多杂质的洗脱溶剂配比。【方法】通过改变正己烷（H）与二氯甲烷（D）体积比来调节洗脱液极性，按极性从小到大对标准样品进行洗脱，并计算每种烯酮分子的洗脱量。【结果】当VH∶VD=8∶1时烯酮开始被洗脱出，到1∶2时可被完全洗脱。烯酮C38：2 Et最先被洗脱出，而C37：3最后被洗脱出。各烯酮分子被洗脱量的峰值出现在不同极性的洗脱液，按极性排序为C38：2 Et < C38：3 Et < （C38：2 Me、C37：2）< C37：3。【结论】洗脱液极性对不同烯酮分子洗脱效果有差异，如果C37洗脱不完全，可能会使偏大，导致换算的温度偏高。VH∶VD小于1∶2时，烯酮分子可被完全洗脱。
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