2022, 42(5): 54-61. doi: 10.3969/j.issn.1673-9159.2022.05.007
关键词： 氮源 , 湛江等鞭金藻 , 配方优化 , 化肥尿素 , 生长特性 , 生产成本
Keywords： nitrogen source , Isochrysis zhanjiangensis , formula optimization , fertilizer urea , growth , cost-production
【目的】探寻适合湛江等鞭金藻规模化低成本培养的氮源。【方法】以宁波3#微藻培养液为基础培养基，分别以不同质量浓度的无机氮［尿素（35.33 mg·L-1）、化肥尿素（复合肥）（76.14 mg·L-1）、氯化铵（62.94 mg·L-1）、乙酸铵（90.95 mg·L-1）］和有机氮［胰蛋白胨（63.54 mg·L-1）］为氮源供体，研究不同氮源供体对湛江等鞭金藻培养效果的影响，实验将接种藻密度控制为10.0×104 mL-1，培养周期为21 d。【结果】以化肥尿素为氮源供体培养下湛江等鞭金藻生产力、比生长速率、藻密度、叶绿素a、叶绿素b和总叶绿素质量浓度显著优于硝酸钠、尿素、氯化铵、乙酸铵和胰蛋白胨（P < 0.05）；培养水体中化肥尿素总氮和总磷的吸收率分别为94.2%和99.0%。【结论】化肥尿素氮源供体是培养湛江等鞭金藻最优质的培养基配方氮源，显著优于硝酸钠和其他氮源供体（P < 0.05）。
【Objective】To study the nitrogen source formula suitable for the large-scale and low-cost culture of Isochrysis zhanjiangensis.【Method】Ningbo 3#microalgae culture medium was used as the basic culture medium, and different concentrations of inorganic nitrogen [urea(35.33 mg·L-1), chemical fertilizer urea (compound fertilizer) (76.14 mg·L-1), NH4Cl (62.94 mg·L-1), CH3COONH4 (90.95 mg·L-1)]and organic nitrogen [tryptone(63.54 mg·L-1)] were used as nitrogen source donors respectively.The effects of different nitrogen source donors on the culture effect of I.zhanjiangensis were studied.The density of inoculated algae was 10.0×104 mL-1 and the cultivation experiment was lasted for 21 d.【Result】The productivity, specific growth rate, algal density, chlorophyll a, chlorophyll b and total chlorophyll mass concentration of I.zhanjiangensis cultured with fertilizer urea as nitrogen source were significantly better than those of NaNO3, NH2CONH2, NH4Cl, CH3COONH4 and tryptone (P < 0.05); The absorption rates of total nitrogen and total phosphorus of fertilizer urea in culture water were 94.2% and 99.0%.【Conclusion】The nitrogen source donor is chemical fertilizer, and urea is the best medium formula for cultivating I.zhanjiangensis, which is significantly better than sodium nitrate and other donor nitrogen source media in this experiment(P < 0.05).
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