首页 >  广东海洋大学学报 >  低温胁迫对军曹鱼幼鱼血清生化指标、肝脏抗氧化酶活性及凋亡相关基因表达量的影响

2022, 42(5): 18-26. doi: 10.3969/j.issn.1673-9159.2022.05.003

低温胁迫对军曹鱼幼鱼血清生化指标、肝脏抗氧化酶活性及凋亡相关基因表达量的影响

1. 广东海洋大学水产学院, 广东 湛江 524088;

2. 广东蓝粮种业有限公司, 广东 湛江 524000;

3. 广东省水产动物病害防控与健康养殖重点实验室, 广东 湛江 524088

收稿日期:2022-05-13
修回日期:2022-05-13

基金项目:   财政部和农业农村部国家现代农业产业技术体系专项资金资助(CARS-47);国家重点研发计划项目(2020YFD0900200);广东省科技计划项目(2016B020201009) 

关键词: 军曹鱼 , 低温胁迫 , 生理生化指标 , 抗氧化酶 , 凋亡相关基因

Effects of Low-Temperature Stress on Serum Biochemical, Antioxidant Enzymes Activities and Apoptosis-Related Gene Expression in Liver of Juvenile Cobia(Rachycentron canadum)

1. College of Fisheries, Guangdong Ocean University, Zhanjiang 524088, China;

2. Guangdong Blue Granary Seed Industry Co., Ltd, Zhanjiang 524000, China;

3. Guangdong Provincial Key Laboratory of Aquatic Animal Disease Control and Healthy Culture, Zhanjiang 524088, China

Received Date:2022-05-13
Accepted Date:2022-05-13

Keywords: Rachycentron canadum , low-temperature stress , biochemical index , antioxidant enzyme , apoptosis-related gene

【目的】探究低温对军曹鱼(Rachycentron canadum)幼鱼生理机能的影响。【方法】将军曹鱼幼鱼分别置于28(对照组)、25、21、18 ℃4 种温度条件下,于0、4、7 d 取样检测血清生化指标、肝脏抗氧化酶活性和凋亡相关基因表达量。【结果】(1)军曹鱼血清葡萄糖(GLU)含量在4、7 d时28℃组显著高于温度处理组(P < 0.05);甘油三酯(TG)含量在0、7 d时21、18 ℃组显著高于28、25 ℃组(P < 0.05);总胆固醇(T-CHO)含量在21、18 ℃组所有时间点均显著低于28、25 ℃组(P < 0.05);总蛋白(TP)含量仅在7 d 时21、18 ℃组显著低于28、25 ℃组(P < 0.05);谷丙转氨酶(ALT)活性在0、4 d时18 ℃组含量显著高于其他组(P < 0.05);谷草转氨酶(AST)活性在0 d时28 ℃组显著低于温度处理组(P < 0.05);碱性磷酸酶(AKP)在4、7 d时21、18 ℃组与28、25 ℃组有显著差异(P < 0.05)。(2)肝脏超氧化物歧化酶(SOD)和过氧化氢酶(CAT)活性在4、7 d 时21、18 ℃组显著低于28、25 ℃组(P < 0.05);谷胱甘肽过氧化物酶(GPx)活性在4 d 时21、18 ℃组显著低于28、25 ℃组(P < 0.05),在7 d 时则显著升高(P < 0.05)。丙二醛(MDA)含量21℃和18℃组均高于28、25 ℃组且在7 d时差异显著(P < 0.05)。(3)肝脏凋亡相关基因bax、caspase-9、caspase-3、p53、mdm2 表达量在温度处理组中均高于28 ℃组,Bcl-2 在0、7 d 时18 ℃组显著低于其他组(P < 0.05)。【结论】低温胁迫显著降低军曹鱼幼鱼血清抗氧化相关酶活性,诱导肝脏组织中凋亡相关基因表达,从而造成氧化损伤,促进细胞凋亡。

【Objective】To investigate the effects of low-temperature stress on physiological function of juvenile cobia (Rachycentron canadum), 【Method】Juvenile cobia were put in four aquaculture tanks whose water temperatures were 28 ℃(control), 25 ℃, 21 ℃and 18 ℃respectively to study the changes of serum biochemical, antioxidant enzyme activities and apoptosis-related gene expression in the liver of cobia at different time (0, 4 and 7 d).【Results】The results in the current study indicated that:(1) The serum glucose (GLU) content of cobia at 4 and 7 d in the 28 ℃group was significantly higher than that in temperature treatment groups (P < 0.05); The content of triglyceride (TG) was extensively higher in the 21 and 18 ℃groups than in the 28 and 25 ℃groups (P < 0.05) at 0 and 7 d; Compared with the 28 and 25 ℃groups, the total cholesterol (T-CHO) content showed a significant decrease in the 21 and 18 ℃groups (P < 0.05) at 0, 4 and 7 d.The total protein (TP) content considerably lowered in the 21 and 18 ℃groups, compared to the 28 and 25 ℃groups after 7 d (P < 0.05).The alanine aminotransferase (ALT) activity was considerably higher in the 18 ℃group than other groups at 0 and 4 d, while the activity of aspartate aminotransferase (AST) in the 28 ℃group decreased significantly versus temperature treatment groups at 0 d (P < 0.05).There were significant variations in serum alkaline phosphatase (AKP) between the two groups (21 and 18 ℃) and the other two groups (28 and 25 ℃) at the times of 4 and 7 d (P < 0.05).(2) The activities of superoxide dismutase (SOD) and catalase (CAT) in the 21 and 18 ℃groups were significantly lower than those in the 28 and 25 ℃groups at 4 and 7 d in the liver (P < 0.05).Hepatic glutathione peroxidase (GPx)activity in the 21 and 18 ℃groups significantly decreased at 4 d, but significantly increased at 7 d, compared to the 28 and 25 ℃groups (P < 0.05).The content of malondialdehyde (MDA) was significantly higher in the 21 and 18 ℃groups than that in the 28 and 25 ℃groups at 7 d (P < 0.05).(3) The expression levels of hepatic apoptosis-related genes (i.e. bax, caspase-9, caspase-3, p53 and mdm2) up-regulated in the 28 ℃group than temperature treatment groups, while bcl-2 down-regulated considerably in the 18 ℃group, compared to other groups at 0 and 7 d (P < 0.05).【Conclusion】Low-temperature stress could significantly lower the activities of antioxidation-related enzymes and induce the expression of apoptosis-related genes, thereby leading to the oxidative damage and the promotion of cellular apoptosis in cobia.

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目录

低温胁迫对军曹鱼幼鱼血清生化指标、肝脏抗氧化酶活性及凋亡相关基因表达量的影响