首页 >  广东海洋大学学报 >  不同生长速率花鲈肌肉转录组分析及生长相关基因筛选

2022, 42(5): 9-17. doi: 10.3969/j.issn.1673-9159.2022.05.002

不同生长速率花鲈肌肉转录组分析及生长相关基因筛选

1. 中国水产科学研究院南海水产研究所/广东省渔业生态环境重点实验室, 广东 广州 510300;

2. 广东省农业技术推广中心, 广东 广州 510520;

3. 上海海洋大学水产与生命学院, 上海 201306

收稿日期:2022-01-05
修回日期:2022-01-05

基金项目:   国家自然科学基金(31802281);广东省重点领域研发计划项目(2021B0202020002);中国水产科学研究院中央级公益性科研院所基本科研业务费专项资金(2020TD21);广州市科技计划项目(202102020487);广州市农村科技特派员项目(20212100051);中国水产科学研究院南海水产研究所中央级公益性科研院所基本科研业务费专项资金(2021TS03;2021SD11) 

关键词: 花鲈 , 转录组 , 差异表达基因 , GO富集 , KEGG富集

Muscle Transcriptome Analysis and Growth-Related Genes Screening of Lateolabrax maculatus with Different Growth Rate

1. Guangdong Provincial Key Laboratory of Fishery Ecology and Environment, South China Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Guangzhou 510300, China;

2. Guangdong Agricultural Technology Extension Centre, Guangzhou 510520, China;

3. College of Aqua-life Science and Technology, Shanghai Ocean University, Shanghai 201306, China

Received Date:2022-01-05
Accepted Date:2022-01-05

Keywords: Lateolabrax maculatus , transcriptome , differentially expressed genes , GO enrichment , KEGG enrichment

摘要

【目的】筛选与花鲈肌肉生长相关的基因和信号通路,为揭示花鲈肌肉生长发育的遗传机制提供理论依据。【方法】分别取4尾生长快速[体长(34.22±2.53)cm]和4尾生长慢速[体长(17.46±1.78)cm]的花鲈个体肌肉进行转录组测序。用DESeq2 R 软件包筛选差异表达基因(Differentially expressed genes,DEGs),对DEGs进行GO富集和KEGG富集分析。通过实时荧光定量PCR验证转录组数据的准确性。【结果与结论】测序原始数据经过质量控制和组装共得到137 960 个unigenes,其中68 334 个unigenes 在Nr 中注释成功。获得10 552 个DEGs,其中2 064个DEGs表达量上调,8 488个DEGs表达量下调,鉴定出胰岛素样生长因子结合蛋白1基因(IGFBP1)、纤维细胞生长因子基因(FGF)、肌肉生长抑制素基因(MSTN)、生长激素受体1 基因(GHR1)等与肌肉生长发育相关的基因。DEGs 的GO 富集结果显示,与物质和能量代谢相关的条目差异显著。KEGG 富集结果显示,糖酵解/合成、腺苷酸活化蛋白激酶(AMPK)、脂肪酸降解、精氨酸和脯氨酸代谢等信号通路与肌肉生长密切相关。

【目的】筛选与花鲈肌肉生长相关的基因和信号通路,为揭示花鲈肌肉生长发育的遗传机制提供理论依据。【方法】分别取4尾生长快速[体长(34.22±2.53)cm]和4尾生长慢速[体长(17.46±1.78)cm]的花鲈个体肌肉进行转录组测序。用DESeq2 R 软件包筛选差异表达基因(Differentially expressed genes,DEGs),对DEGs进行GO富集和KEGG富集分析。通过实时荧光定量PCR验证转录组数据的准确性。【结果与结论】测序原始数据经过质量控制和组装共得到137 960 个unigenes,其中68 334 个unigenes 在Nr 中注释成功。获得10 552 个DEGs,其中2 064个DEGs表达量上调,8 488个DEGs表达量下调,鉴定出胰岛素样生长因子结合蛋白1基因(IGFBP1)、纤维细胞生长因子基因(FGF)、肌肉生长抑制素基因(MSTN)、生长激素受体1 基因(GHR1)等与肌肉生长发育相关的基因。DEGs 的GO 富集结果显示,与物质和能量代谢相关的条目差异显著。KEGG 富集结果显示,糖酵解/合成、腺苷酸活化蛋白激酶(AMPK)、脂肪酸降解、精氨酸和脯氨酸代谢等信号通路与肌肉生长密切相关。

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目录

不同生长速率花鲈肌肉转录组分析及生长相关基因筛选