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2016, 36(4): 17-22. doi: 10.3969/j.issn.1673-9159.2016.04.004

基于线粒体COI基因的雷州半岛马尾藻属常见种DNA条形码鉴定及系统进化分析

广东海洋大学水产学院 南海水产经济动物增养殖广东普通高校重点实验室, 广东 湛江 524025

收稿日期:2016-06-07

基金项目:   2016年度广东大学生科技创新培育专项资金立项项目(pdjh2016b0236);中央分成海域使用金支出项目(2011-2-2-09-3);广东省海洋渔业科技推广专项(A201308E02);广东省科技计划项目(2012B020307006) 

关键词: 马尾藻属 , mtDNA-COI基因 , 物种鉴定 , 分子系统进化 , DNA条形码

DNA Barcoding and Phylogenetic Analysis of Common Species of Sargassum from Leizhou Peninsula Based on mtDNA- COI Gene Sequences

Key Laboratory of Aquaculture in South China Sea for Aquatic Economic Animal of Guangdong Higher Education Institutes, Fisheries College, Guangdong Ocean University, Zhanjiang 524025, China

Received Date:2016-06-07

Keywords: Sargassum , mtDNA-COI , species identification , molecular phylogenetic , DNA barcoding

采用DNA条形码技术辅以传统形态分类方法,对雷州半岛马尾藻属(Sargassum)6常见种进行物种鉴定和系统进化分析。测得6种马尾藻线粒体COI基因序列,该序列与GenBank和BOLD数据库中马尾藻序列同源性均大于或等于99%,该结果与形态分类学鉴定结果一致。COI基因序列特征分析表明,6种马尾藻保守位点421个,变异位点58个,简约信息位点21个,单一突变位点37个,变异率7.7%,T、C、A、G平均含量分别为39.1%、18.9%、19.1%、22.9%,A+T含量(58.2%)高于C+G含量(41.8%),UUU所编码的苯丙氨酸(F)使用频率最高,达12.5%,最大似然法估算的转换颠换比值为2.42,种间遗传距离多在0.030±0.013~0.100±0.016之间。聚类分析结果与形态学鉴定、同源性分析结果一致。

Species identification and phylogenetic relationship in 6 common species of Sargassum collected from Leizhou Peninsula were analyzed by appling DNA barcoding technology assisted traditional morphological classification method. Mt-DNA COI gene sequences of 6 species of Sargassum and existing data in GenBank and BOLD database for homology analysis showed that the similarity is 99% or more, the molecular data were consistent with the morphological identification results. In addition, the analyses on characteristics of COI gene sequence shows that COI gene sequence in 6 species Sargassum including 421 conserved sites, 58 variable sites, 21 Parsim-Informative sites, and 37 Singleton sites, accounted for 7.7%. Nucleartides composition results showed:T, C, A, G average contents were 39.1%, 18.9%, 19.1%, 22.9%, respectively, and A+T content (58.2%) was significantly higher than C+G content (41.8%). The highest frequency of amino acid was Phenylalanine (F) which coded by UUU(12.5%). The rate of transition and transversion based on maximum likelihood was 2.42. Interspecific genetic distances were from 0.001±0.001 to 0.113±0.016. The result of cluster analysis was consistent with that of morphological identification and homology analysis.

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基于线粒体COI基因的雷州半岛马尾藻属常见种DNA条形码鉴定及系统进化分析