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ISSN 2095-1388

主管 辽宁省教育厅

主办 大连海洋大学

急性温度胁迫对虹鳟肝脏代谢酶活性及生长相关基因表达的影响

2014. 急性温度胁迫对虹鳟肝脏代谢酶活性及生长相关基因表达的影响. 大连海洋大学学报, 29(6): 566-571. doi: 10.3969/J.ISSN.2095-1388.2014.06.005
引用本文: 2014. 急性温度胁迫对虹鳟肝脏代谢酶活性及生长相关基因表达的影响. 大连海洋大学学报, 29(6): 566-571. doi: 10.3969/J.ISSN.2095-1388.2014.06.005
GUAN Biao, WEN Hai-shen, LIU Qun, WANG Jin-huan, WANG Qing-long. 2014. Effects of acute temperature stress on metabolic enzyme activity and gene expression related to growth in rainbow trout Oncorhynchus mykiss. Journal of Dalian Ocean University, 29(6): 566-571. doi: 10.3969/J.ISSN.2095-1388.2014.06.005
Citation: GUAN Biao, WEN Hai-shen, LIU Qun, WANG Jin-huan, WANG Qing-long. 2014. Effects of acute temperature stress on metabolic enzyme activity and gene expression related to growth in rainbow trout Oncorhynchus mykiss. Journal of Dalian Ocean University, 29(6): 566-571. doi: 10.3969/J.ISSN.2095-1388.2014.06.005

急性温度胁迫对虹鳟肝脏代谢酶活性及生长相关基因表达的影响

  • 基金项目:

    国家公益性行业(农业)科研专项(201003055)

详细信息
    作者简介:

    管标(1988-),男,硕士研究生。E-mail:guanbiao5258@126.com

    通讯作者: , E-mail: wenhaishen@ouc.edu.cn
  • 中图分类号: S917.4

Effects of acute temperature stress on metabolic enzyme activity and gene expression related to growth in rainbow trout Oncorhynchus mykiss

  • Fund Project: 国家公益性行业(农业)科研专项(201003055)
More Information
  • 为探究急性温度胁迫对虹鳟Oncorhynchus mykiss肝脏代谢酶和部分生长调控基因表达的影响,采用血清生化分析仪测定了低温(6℃)和高温(24℃)胁迫下虹鳟(体质量为240 g±18 g)血清中谷草转氨酶(AST)、谷丙转氨酶(ALT)和碱性磷酸酶(ALP)的活性,通过放射免疫法测定了血清类胰岛素生长因子1(IGF-1)的含量,采用实时定量法测定了肝脏中GHR1、IGF-1和IGF-2的表达量。结果表明:温度胁迫2 h后,低温和高温两个处理组鱼的AST活性和高温组的ALT活性均呈先升高后降低的趋势,均在恢复6 h时达到最大值,而低温组鱼的ALT活性在胁迫后恢复0 h时就显著升高(P<0.05),随后逐渐降低至对照组水平;低温组鱼的ALP活性在胁迫后显著降低(P<0.05),并在48 h的恢复期间始终维持在较低水平,而高温组鱼的ALP活性比对照组略有降低(P>0.05);低温组鱼的血清IGF-1激素含量在恢复12 h后呈上升趋势,并在48 h时恢复至对照组水平,而高温组血清IGF-1含量在胁迫后始终处于较低水平;两个处理组肝脏中,GHR1基因表达量比对照组略有降低(P>0.05),而IGF-1和IGF-2基因表达量均呈先降低后升高的趋势,且IGF-2表达量下降更明显,并在恢复12 h时达到最低值。研究表明:温度胁迫后,虹鳟血清转氨酶活性降低,肝脏受到损伤;血清IGF-1激素含量下降,肝脏中GHR1、IGF-1和IGF-2基因表达量均低于对照组;高温组各指标变化较大,说明属于冷水鱼类的虹鳟受高温影响更为显著。
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收稿日期:  2014-03-29

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