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ISSN 1005-8737

主管 中华人民共和国农业部

主办 中国水产科学研究院,中国水产学会,中国科技出版传媒股份有限公司

半滑舌鳎骨形态发生蛋白4基因的克隆和表达分析

2016. 半滑舌鳎骨形态发生蛋白4基因的克隆和表达分析. 中国水产科学, 23(3): 500-512. doi: 10.3724/SP.J.1118.2016.15346
引用本文: 2016. 半滑舌鳎骨形态发生蛋白4基因的克隆和表达分析. 中国水产科学, 23(3): 500-512. doi: 10.3724/SP.J.1118.2016.15346
MA Qian, FENG Wenrong, LIU Shufang, ZHUANG Zhimeng, MA Hui. 2016. Molecular cloning and expression analysis of BMP4 gene in tongue sole (Cynoglossus semilaevis). Journal of Fishery Sciences of China, 23(3): 500-512. doi: 10.3724/SP.J.1118.2016.15346
Citation: MA Qian, FENG Wenrong, LIU Shufang, ZHUANG Zhimeng, MA Hui. 2016. Molecular cloning and expression analysis of BMP4 gene in tongue sole (Cynoglossus semilaevis). Journal of Fishery Sciences of China, 23(3): 500-512. doi: 10.3724/SP.J.1118.2016.15346

半滑舌鳎骨形态发生蛋白4基因的克隆和表达分析

  • 基金项目:

    国家自然科学基金项目(31201981)

    中国水产科学研究院黄海水产研究所级基本科研业务费资助项目(20603022012032).

详细信息
    作者简介:

    马骞(1983-), 女, 博士, 研究方向为渔业资源分子生态学. E-mail: maqian@ysfri.ac.cn

  • 中图分类号: S917

Molecular cloning and expression analysis of BMP4 gene in tongue sole (Cynoglossus semilaevis)

  • Fund Project: 中国水产科学研究院黄海水产研究所级基本科研业务费资助项目(20603022012032).
  • 本研究克隆了半滑舌鳎(Cynoglossus semilaevis)骨形态发生蛋白4(BMP4)基因的cDNA序列,并分析其在半滑舌鳎成鱼的组织表达分布及其在早期发育阶段的定位和表达变化趋势。结果表明,半滑舌鳎BMP4基因cDNA全长为1680 bp,包括了419 bp的5'非翻译区(5'UTR)、编码403个氨基酸的1212 bp的开放阅读框(ORF)以及49 bp的3'非翻译区(3'UTR)。同源性分析和分子进化聚类分析结果显示,半滑舌鳎与已报道慈鲷科(Cichlidae)各鱼种的同源性最高,并与之共同聚为鱼类BMP4分支。BMP4 mRNA在半滑舌鳎成鱼的13个组织中具有广泛的分布,并在鳃组织中表达量最高,其次在背鳍、软骨等组织具有中等水平的表达。此外, BMP4基因在早期胚胎发育阶段(卵裂期和原肠期)具有极高水平的表达,此后其表达水平逐渐降低;但在后期仔鱼期(孵化后3日龄和4日龄),其表达水平再次升高。整体原位杂交检测结果表明, BMP4 mRNA在早期仔鱼期主要在头部及躯干前部表达;后期仔鱼BMP4基因主要在冠状幼鳍、上下颌及胸鳍处表达;进入稚鱼期后在鳃盖骨及各鳍均有表达。上述研究结果揭示了BMP4在半滑舌鳎早期骨骼发育中的重要作用,为揭示海水硬骨鱼类骨骼发生、发育的调控机制奠定了理论基础。
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出版历程
收稿日期:  2015-09-07
修回日期:  2015-11-24

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