Killing effect of ozone on Aeromonas hydrophila in aquaculture tailwater
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摘要: 为改善臭氧消毒工艺，优化养殖尾水高效消毒技术，利用臭氧发生器及控制发生时间制备出不同浓度的臭氧水，采用悬液定量法分别测定了不同浓度臭氧水在不同胁迫时间下对嗜水气单胞菌（Aeromonas hydrophila）的杀灭效果，并与常见消毒剂过硫酸氢钾进行了灭菌效果比较，同时测试了臭氧处理含菌养殖尾水的实际效果。结果表明：当嗜水气单胞菌浓度为6.4×105、7.5×106、1.5×107、3.2×108 CFU/mL时，用1.2 mg/L的臭氧水作用2 min时，灭菌率分别为92.66%、84.00%、80.67%、79.58%，作用6 min时灭菌率分别为100%、99.72%、99.96%、99.98%，而用400 mg/L的过硫酸氢钾作用6 min时灭菌率才达到100%；用1.2 mg/L臭氧水对含菌浓度为2.3×103、2.7×105 CFU/mL的养殖尾水作用 6 min，灭菌率皆达到100%。研究表明，臭氧消毒灭菌效率远高于过硫酸氢钾，臭氧水在最佳质量浓度为1.2 mg/L、作用时间为6 min时，能够高效杀灭嗜水气单胞菌，其灭菌率与含菌量、臭氧质量浓度和作用时间相关。Abstract: To improve ozone disinfection process and optimize high efficiency disinfection technology of tailwater in aquaculture, different concentrations of ozonated water were prepared by regulation of generation period of an ozone generator, concentration and exposure period (1 and 10 min) of ozone neutralizer, and the sterilizing effect of ozonated water against different concentrations of Aeromonas hydrophila was determined by suspension quantification method under different stress times. The sterilizing effect was compared with the conventional disinfectant potassium persulfate. The results showed that the sterilization rate was found to be 92.66% at 6.4×105CFU/mL, 84.00% at 7.5×106CFU/mL, 80.67% at 1.5×107 CFU/mL and 79.58% at 3.2×108 CFU/mL with 1.2 mg/L ozone for 2 min, and 100%, 99.72%, 99.96% and 99.98% for 6 min, while the sterilization rate was 100% when potassium persulfate at 400 mg/L was used for 6 min, and the actual effects of ozone treatment of bacterial culture tail water were also tested. The findings indicated that the sterilization efficiency of ozone was much higher than that of potassium persulfate, with the optimal concentration of 1.2 mg/L, and the action time of 6 min, indicating that ozone sterilization rate for A.hydrophila was correlated with number of bacteria, concentration of ozone, and active time.
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Key words:
- ozone disinfection /
- Aeromonas hydrophila /
- aquaculture tailwater /
- killing effect
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