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ISSN 2095-1388

主管 辽宁省教育厅

主办 大连海洋大学

皱纹盘鲍肽聚糖识别蛋白的表达及免疫功能特性

2023. 皱纹盘鲍肽聚糖识别蛋白的表达及免疫功能特性. 大连海洋大学学报, 38(6): 947-955. doi: 10.16535/j.cnki.dlhyxb.2023-111
引用本文: 2023. 皱纹盘鲍肽聚糖识别蛋白的表达及免疫功能特性. 大连海洋大学学报, 38(6): 947-955. doi: 10.16535/j.cnki.dlhyxb.2023-111
QIAO Kun, ZHENG Chunhua, CHEN Bei, SU Yongchang, HAO Hua, XU Min, CAI Shuilin, LIU Zhiyu. 2023. Expression and immune function characteristics of peptidoglycan recognition protein gene in disk abalone (Haliotis discus hannai). Journal of Dalian Ocean University, 38(6): 947-955. doi: 10.16535/j.cnki.dlhyxb.2023-111
Citation: QIAO Kun, ZHENG Chunhua, CHEN Bei, SU Yongchang, HAO Hua, XU Min, CAI Shuilin, LIU Zhiyu. 2023. Expression and immune function characteristics of peptidoglycan recognition protein gene in disk abalone (Haliotis discus hannai). Journal of Dalian Ocean University, 38(6): 947-955. doi: 10.16535/j.cnki.dlhyxb.2023-111

皱纹盘鲍肽聚糖识别蛋白的表达及免疫功能特性

  • 基金项目:

    厦门市青年创新基金(3502Z20206002);福建省种业创新与产业化工程项目(2021FJSCZY02);福建省促进海洋与渔业产业高质量发展专项资金项目(FJHYF-L-2023-23);厦门市海洋与渔业发展专项资金资助项目(21CZP006HJ04)

详细信息
    作者简介:

    乔琨(1983-),女,博士,助理研究员。E-mail:qiaokun@xmu.edu.cn

  • 中图分类号: S 917.4

Expression and immune function characteristics of peptidoglycan recognition protein gene in disk abalone (Haliotis discus hannai)

  • Fund Project: 厦门市青年创新基金(3502Z20206002);福建省种业创新与产业化工程项目(2021FJSCZY02);福建省促进海洋与渔业产业高质量发展专项资金项目(FJHYF-L-2023-23);厦门市海洋与渔业发展专项资金资助项目(21CZP006HJ04)
  • 为研究皱纹盘鲍(Haliotis discus hannai)肽聚糖识别蛋白HdhPGRP-SC2-like的免疫功能特性, 利用RACE技术克隆了HdhPGRP-SC2-like基因的全长cDNA序列,采用实时荧光定量PCR(qPCR)分析了HdhPGRP-SC2-like基因的组织分布特征,通过原核表达获得了重组蛋白rHdhPGRP-SC2-like, 并对其酰胺酶活性及免疫特性进行了验证。结果表明:HdhPGRP-SC2-like基因的cDNA全长为938 bp,开放阅读框为780 bp,编码260个氨基酸,包含保守的PGRP结构域和Ami_2结构域;HdhPGRP-SC2-like属于短型PGRP,与软体动物近缘物种PGRP具有高度相似性;qPCR分析显示,HdhPGRP-SC2-like mRNA在皱纹盘鲍各组织中均有表达,其中,鳃中表达量最高,外套膜、消化腺中表达量次之,腹足和血细胞中表达量较低;进一步构建原核表达载体pET-28a(+)-HdhPGRP-SC2-like,经诱导表达、分离纯化获得重组蛋白rHdhPGRP-SC2-like,该蛋白具有肽聚糖结合活性,且在Zn2+存在的情况下显示出酰胺酶活性。研究表明,HdhPGRP-SC2-like能够结合细菌肽聚糖成分,在机体抵御入侵细菌等免疫防御中发挥重要作用。
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出版历程
收稿日期:  2023-05-12

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