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ISSN 2095-1388

主管 辽宁省教育厅

主办 大连海洋大学

外源添加胞外囊泡对海假交替单胞菌生物被膜形成及厚壳贻贝附着的影响

2023. 外源添加胞外囊泡对海假交替单胞菌生物被膜形成及厚壳贻贝附着的影响. 大连海洋大学学报, 38(6): 994-1002. doi: 10.16535/j.cnki.dlhyxb.2023-097
引用本文: 2023. 外源添加胞外囊泡对海假交替单胞菌生物被膜形成及厚壳贻贝附着的影响. 大连海洋大学学报, 38(6): 994-1002. doi: 10.16535/j.cnki.dlhyxb.2023-097
WU Guanju, MIAO Tianyin, HU Xiaomeng, ZHANG Wen, YANG Jinlong, LIANG Xiao. 2023. Effects of exogenous addition of outer membrane vesicles on biofilm formation of Pseudoalteromonas marina and settlement of mussel (Mytilus coruscus). Journal of Dalian Ocean University, 38(6): 994-1002. doi: 10.16535/j.cnki.dlhyxb.2023-097
Citation: WU Guanju, MIAO Tianyin, HU Xiaomeng, ZHANG Wen, YANG Jinlong, LIANG Xiao. 2023. Effects of exogenous addition of outer membrane vesicles on biofilm formation of Pseudoalteromonas marina and settlement of mussel (Mytilus coruscus). Journal of Dalian Ocean University, 38(6): 994-1002. doi: 10.16535/j.cnki.dlhyxb.2023-097

外源添加胞外囊泡对海假交替单胞菌生物被膜形成及厚壳贻贝附着的影响

  • 基金项目:

    上海市优秀学术带头人项目(20XD1421800);国家自然科学基金(41876159);国家重点研发计划(2022YFE0204600)

详细信息
    作者简介:

    吴冠举(1997-),男,硕士研究生。E-mail:m200100020@st.shou.edu.cn

  • 中图分类号: S 968.3

Effects of exogenous addition of outer membrane vesicles on biofilm formation of Pseudoalteromonas marina and settlement of mussel (Mytilus coruscus)

  • Fund Project: 上海市优秀学术带头人项目(20XD1421800);国家自然科学基金(41876159);国家重点研发计划(2022YFE0204600)
  • 为探究胞外囊泡(outer membrane vesicles,OMVs)对海假交替单胞菌(Pseudoalteromonas marina)生物被膜形成及厚壳贻贝(Mytilus coruscus)(壳长为0.88 mm±0.16 mm,壳高为0.55 mm±0.12 mm)稚贝附着的影响,从海假交替单胞菌中提取并纯化了OMVs,并添加到海假交替单胞菌中共同孵育形成生物被膜,在OMVs终质量浓度为0.1、1、10、20 μg/mL时检测形成的生物被膜对贻贝稚贝附着的诱导作用。结果表明:在海假交替单胞菌中成功提取到了OMVs,从菌体中提取获得的OMVs较上清液中更多;添加10 μg/mL的OMVs时,形成的生物被膜对贻贝稚贝的附着诱导活性最高,稚贝附着率达到56.42%;随着OMVs添加量的增加,细菌密度和膜厚呈增长趋势,胞外产物中α-多糖、β-多糖、脂质和蛋白质含量呈升高趋势;相关性分析显示,生物被膜中的脂质含量与贻贝附着显著相关(P<0.05)。研究表明,OMVs的添加可促进海假交替单胞菌生物被膜形成,提高生物被膜胞外产物的含量,并推测主要通过增加脂质含量提高生物被膜对贻贝附着的诱导能力。
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出版历程
收稿日期:  2023-04-25

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