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ISSN 2095-1388

主管 辽宁省教育厅

主办 大连海洋大学

斑马鱼hoxb3a基因在早期心脏发育中的功能分析

2023. 斑马鱼hoxb3a基因在早期心脏发育中的功能分析. 大连海洋大学学报, 38(6): 956-963. doi: 10.16535/j.cnki.dlhyxb.2023-089
引用本文: 2023. 斑马鱼hoxb3a基因在早期心脏发育中的功能分析. 大连海洋大学学报, 38(6): 956-963. doi: 10.16535/j.cnki.dlhyxb.2023-089
WANG Bingqi, HE Hongyang, WANG Jie, ZU Yao. 2023. Function analysis of zebrafish (Danio rerio) hoxb3a gene in early heart development. Journal of Dalian Ocean University, 38(6): 956-963. doi: 10.16535/j.cnki.dlhyxb.2023-089
Citation: WANG Bingqi, HE Hongyang, WANG Jie, ZU Yao. 2023. Function analysis of zebrafish (Danio rerio) hoxb3a gene in early heart development. Journal of Dalian Ocean University, 38(6): 956-963. doi: 10.16535/j.cnki.dlhyxb.2023-089

斑马鱼hoxb3a基因在早期心脏发育中的功能分析

  • 基金项目:

    国家自然科学基金(32170423,31501166);上海市科委扬帆计划(15YF1405000)

详细信息
    作者简介:

    王冰琦(1998-),女,硕士研究生。E-mail:m200100094@st.shou.edu.cn

  • 中图分类号: S 917.4

Function analysis of zebrafish (Danio rerio) hoxb3a gene in early heart development

  • Fund Project: 国家自然科学基金(32170423,31501166);上海市科委扬帆计划(15YF1405000)
  • 为探究hoxb3a基因在斑马鱼(Danio rerio)心脏早期发育中的调控作用,利用基因沉默技术(Morpholino)对斑马鱼胚胎进行hoxb3a基因敲降,并结合显微镜观察分析、实时荧光定量PCR、原位杂交技术分析hoxb3a基因对斑马鱼心脏发育的影响。结果表明:hoxb3a基因敲降组(hoxb3a morphant)出现心包腔肿大、心脏环化角度异常和心房心室形态改变等现象;原位杂交试验确定了hoxb3a基因在不同时空的表达区域,其表达位置的变化与神经嵴细胞参与心脏发育的形成有关;在受精后3 d,与对照组相比,hoxb3a基因敲降组胚胎一些心脏关键基因的表达量发生变化,其中心脏祖细胞相关基因nkx2.5、gata4及房室形成关键基因nppa的表达量均具有显著升高的趋势,同时与神经嵴发育相关的fgf8a基因表达量也明显升高。研究表明,hoxb3a基因在斑马鱼胚胎的早期心脏发育中,通过调控nkx2.5、gata4、nppafgf8a等相关转录因子的表达,参与斑马鱼早期心脏发育的过程,本研究结果为早期心脏发育研究提供了新思路。
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  • GITTENBERGER-DE GROOT A C, CALKOEN E E, POELMANN R E, et al.Morphogenesis and molecular considerations on congenital cardiac septal defects[J].Annals of Medicine, 2014, 46(8):640-652.

    BRUNEAU B G.The developmental genetics of congenital heart disease[J].Nature, 2008, 451(7181):943-948.

    BUCKINGHAM M, MEILHAC S, ZAFFRAN S.Building the mammalian heart from two sources of myocardial cells[J].Nature Reviews Genetics, 2005, 6(11):826-835.

    VINCENT S D, BUCKINGHAM M E.How to make a heart[J].Current Topics in Developmental Biology, 2010, 90:1-41.

    BERTRAND N, ROUX M, RYCKEBVSCH L, et al.Hox genes define distinct progenitor sub-domains within the second heart field[J].Developmental Biology, 2011, 353(2):266-274.

    AHMAD S M.Conserved signaling mechanisms in Drosophila heart development[J].Developmental Dynamics, 2017, 246(9):641-656.

    TAO Y, SCHULZ R A.Heart development in Drosophila[J].Seminars in Cell & Developmental Biology, 2007, 18(1):3-15.

    VIEUX-ROCHAS M, MASCREZ B, KRUMLAUF R, et al.Combined function of HoxA and HoxB clusters in neural crest cells[J].Developmental Biology, 2013, 382(1):293-301.

    MANLEY N R, CAPECCHI M R.Hox group 3 paralogs regulate the development and migration of the thymus, thyroid, and parathyroid glands[J].Developmental Biology, 1998, 195(1):1-15.

    BOUCHERAT O, MONTARON S, BÉRUBÉ-SIMARD F A, et al.Partial functional redundancy between Hoxa5 and Hoxb5 paralog genes during lung morphogenesis[J].American Journal of Physiology:Lung Cellular and Molecular Physiology, 2013, 304(12):L817-L830.

    ZHU S M, YANG Z, ZHANG Z, et al.HOXB3 drives WNT-activation associated progression in castration-resistant prostate cancer[J].Cell Death & Disease, 2023, 14(3):215.

    ZAFFRAN S, KELLY R G.New developments in the second heart field[J].Differentiation, 2012, 84(1):17-24.

    LIU J D, STAINIER D Y R.Zebrafish in the study of early cardiac development[J].Circulation Research, 2012, 110(6):870-874.

    PRUMMEL K D, HESS C, NIEUWENHUIZE S, et al.A conserved regulatory program initiates lateral plate mesoderm emergence across chordates[J].Nature Communications, 2019, 10:3857.

    DEVINE W P, WYTHE J D, GEORGE M, et al.Early patterning and specification of cardiac progenitors in gastrulating mesoderm[J].eLife, 2014, 3:e03848.

    BROWN D R, SAMSA L A, QIAN L, et al.Advances in the study of heart development and disease using zebrafish[J].Journal of Cardiovascular Development and Disease, 2016, 3(2):13.

    HUTSON M R, KIRBY M L.Neural crest and cardiovascular development:a 20-year perspective[J].Birth Defects Research Part C:Embryo Today:Reviews, 2003, 69(1):2-13.

    LIU Y J, CHEN S, ZVHLKE L, et al.Global birth prevalence of congenital heart defects 1970-2017:updated systematic review and meta-analysis of 260 studies[J].International Journal of Epidemiology, 2019, 48(2):455-463.

    LIU Y, WANG C C, GUO Y N, et al.Functional effects of pma-miR-200 c-3 p of sea lamprey on heart development of zebrafish[J].Journal of Dalian Ocean University, 2016, 31(5):510-515.(in Chinese)

    ZHANG H R, XIE J J, SO K K H, et al.Hoxb3 regulates Jag1 expression in pharyngeal epithelium and affects interaction with neural crest cells[J].Frontiers in Physiology, 2021, 11:612230.

    WONG E Y M, WANG X A, MAK S S, et al.Hoxb3 negatively regulates Hoxb1 expression in mouse hindbrain patterning[J].Developmental Biology, 2011, 352(2):382-392.

    MAGNUSSON M, BRUN A C M, LAWRENCE H J, et al.Hoxa9/hoxb3/hoxb4 compound null mice display severe hematopoietic defects[J].Experimental Hematology, 2007, 35(9):1421.e1-1421.e9.

    HADRYS T, PUNNAMOOTTIL B, PIEPER M, et al.Conserved co-regulation and promoter sharing of hoxb3a and hoxb4a in zebrafish[J].Developmental Biology, 2006, 297(1):26-43.

    MACHON O, MASEK J, MACHONOVA O, et al.Meis2 is essential for cranial and cardiac neural crest development[J].BMC Developmental Biology, 2015, 15:40.

    HAMI D, GRIMES A C, TSAI H J, et al.Zebrafish cardiac development requires a conserved secondary heart field[J].Development, 2011, 138(11):2389-2398.

    SOUIDI A, JAGLA K.Drosophila heart as a model for cardiac development and diseases[J].Cells, 2021, 10(11):3078.

    MALLO M.Shaping Hox gene activity to generate morphological diversity across vertebrate phylogeny[J].Essays in Biochemistry, 2022, 66(6):717-726.

    HOLLAND P W H.Evolution of homeobox genes[J].Wiley Interdisciplinary Reviews:Developmental Biology, 2013, 2(1):31-45.

    GAUNT S J.The significance of Hox gene collinearity[J].The International Journal of Developmental Biology, 2015, 59(4/5/6):159-170.

    BLUM M, DE ROBERTIS E M, WALLINGFORD J B, et al.Morpholinos:antisense and sensibility[J].Developmental Cell, 2015, 35(2):145-149.

    KEMMLER C L, RIEMSLAGH F W, MORAN H R, et al.From stripes to a beating heart:early cardiac development in zebrafish[J].Journal of Cardiovascular Development and Disease, 2021, 8(2):17.

    TOVAR J A.The neural crest in pediatric surgery[J].Journal of Pediatric Surgery, 2007, 42(6):915-926.

    SONG M Y, YUAN X F, RACIOPPI C, et al.GATA4/5/6 family transcription factors are conserved determinants of cardiac versus pharyngeal mesoderm fate[J].Science Advances, 2022, 8(10):eabg0834.

    GRASSINI D R, LAGENDIJK A K, DE ANGELIS J E, et al.Nppa and Nppb act redundantly during zebrafish cardiac development to confine AVC marker expression and reduce cardiac jelly volume[J].Development, 2018:dev.160739.

    PAFFETT-LUGASSY N, NOVIKOV N, JEFFREY S, et al.Unique developmental trajectories and genetic regulation of ventricular and outflow tract progenitors in the zebrafish second heart field[J].Development, 2017, 144(24):4616-4624.

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出版历程
收稿日期:  2023-04-21

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