Protective effect of phillygenin (PHI) on liver injury induced by microcystins (MCs) in loach (Paramisgurnus dabryanus)
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摘要: 为探究连翘脂素（phillygenin，PHI）对亚慢性毒性浓度微囊藻毒素（microcystins，MCs）诱发的大鳞副泥鳅（Paramisgurnus dabryanus）肝脏损伤的保护作用，对体质量为（100.13 ±5.23 ）g的泥鳅腹腔注射剂量为17 μg/kg（体质量，下同）MCs 1 d后，分别连续注射3 d不同剂量的PHI（累计剂量0.3、0.6、1.2、2.4 mg/kg），第6天时取泥鳅肝脏样品，比较肝脏组织病理、谷丙转氨酶（ALT）活性、氧化损伤相关指标[丙二醛（MDA）、超氧化物歧化酶（SOD）、谷胱甘肽过氧化物酶（GSH-PX）、谷胱甘肽还原酶（GR）]及炎性因子[白细胞介素1β（IL-1β）和肿瘤坏死因子α（TNF-α）]水平变化。结果表明：MCs可诱导泥鳅肝脏组织发生病理损伤；与对照组相比，MCs组泥鳅ALT活性、MDA含量及IL-1β和TNF-α两种炎性因子水平均显著升高（P<0.05），3种抗氧化酶活性显著降低（P<0.05）；与MCs组相比，注射PHI能减轻MCs诱发的泥鳅肝脏组织病理损伤，显著降低肝脏中ALT活性、MDA含量及IL-1β和TNF-α两种炎性因子水平（P<0.05），显著提高肝脏中SOD、GSH-PX和GR活性（除0.3 mg/kg PHI剂量组外）（P<0.05）。研究表明，PHI能够减轻MCs引起的泥鳅肝脏组织病理与生理功能损伤、氧化损伤和炎症反应，对MCs所致肝脏损伤具有保护作用。Abstract: In order to investigate the protective effect of phillygenin (PHI) on liver injury induced by subchronic toxic concentration of microcystins (MCs), loach (Paramisgurnus dabryanus) with body weight of (100.13±5.23)g were intraperitoneally injected with 17 μg/kg (body weight) MCs on the first day, and then successively injected with different concentrations of PHI [cumulative dose of 0.3, 0.6, 1.2 and 2.4 mg/kg (body weight)] on the following 3 days. On the sixth day, livers of P.dabryanus were taken to compare the histopathologic changes, alanine aminotransferase (ALT) activity, oxidative damage related indices [malonaldehyde (MDA), superoxide dismutase (SOD), glutathione peroxidase (GSH-PX), glutathione reductase (GR)] and inflammatory factors [interleukin 1β (IL-1β) and tumor necrosis factor α (TNF-α)] of liver tissue. It was found that MCs induced histopathologic injury in liver. There was significant increase in ALT activity, the contents of MDA, and IL-1β and (TNF-α) 2 inflammatory factors in MCs group compared with the control group (P<0.05), while there was significant decrease in activities of 3 antioxidant enzymes in MCs group (P<0.05). The injection of PHI alleviated the MCs-induced histopathologic injury in liver, significantly decreased ALT activity, MDA content and 2 inflammatory factors levels (P<0.05), and significantly increased in the activities of SOD, GSH-PX and GR (except 0.3 mg/kg PHI) (P<0.05) compared with the MCs group. PHI alleviated the histopathologic and physiology function injury, oxidative damage and inflammatory response in liver, and had a protective effect on liver injury induced by MCs.
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Key words:
- phillygenin /
- microcystin /
- Paramisgurnus dabryanus /
- liver injury /
- protective effect
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