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ISSN 2095-1388

主管 辽宁省教育厅

主办 大连海洋大学

尼罗罗非鱼生长性状相关微卫星标记的筛选与验证

2023. 尼罗罗非鱼生长性状相关微卫星标记的筛选与验证. 大连海洋大学学报, 38(6): 925-934. doi: 10.16535/j.cnki.dlhyxb.2023-075
引用本文: 2023. 尼罗罗非鱼生长性状相关微卫星标记的筛选与验证. 大连海洋大学学报, 38(6): 925-934. doi: 10.16535/j.cnki.dlhyxb.2023-075
LIU Zhigang, GAO Fengying, TONG Yannan, YI Mengmeng, WANG Miao, ZHU Hai, LU Maixin, KE Xiaoli, CAO Jianmeng. 2023. Screening and verification of microsatellite markers associated with growth traits in Nile tilapia (Oreochromis niloticus). Journal of Dalian Ocean University, 38(6): 925-934. doi: 10.16535/j.cnki.dlhyxb.2023-075
Citation: LIU Zhigang, GAO Fengying, TONG Yannan, YI Mengmeng, WANG Miao, ZHU Hai, LU Maixin, KE Xiaoli, CAO Jianmeng. 2023. Screening and verification of microsatellite markers associated with growth traits in Nile tilapia (Oreochromis niloticus). Journal of Dalian Ocean University, 38(6): 925-934. doi: 10.16535/j.cnki.dlhyxb.2023-075

尼罗罗非鱼生长性状相关微卫星标记的筛选与验证

  • 基金项目:

    广东省重点领域研发计划项目(2021B0202020001);财政部和农业农村部:国家现代农业产业技术体系资助(CARS-46);海南省重点研发计划项目(ZDYF2020096);广东省基础与应用基础研究基金(2021A1515010852);2022年省级乡村振兴战略专项资金种业振兴项目(2022-SPY-00-005)

详细信息
    作者简介:

    刘志刚(1988-),男,博士研究生,助理研究员。E-mail:wenliugang@163.com

  • 中图分类号: S 917.4

Screening and verification of microsatellite markers associated with growth traits in Nile tilapia (Oreochromis niloticus)

  • Fund Project: 广东省重点领域研发计划项目(2021B0202020001);财政部和农业农村部:国家现代农业产业技术体系资助(CARS-46);海南省重点研发计划项目(ZDYF2020096);广东省基础与应用基础研究基金(2021A1515010852);2022年省级乡村振兴战略专项资金种业振兴项目(2022-SPY-00-005)
  • 为筛选尼罗罗非鱼(Oreochromis niloticus)生长相关微卫星分子标记用于指导其选育工作,选用29个微卫星标记对尼罗罗非鱼高要F0群体、高要F1群体、番禺群体和海南群体的生长性状(体质量、全长、体长、头长、体高和体宽)进行了关联性分析和基因型间比较。结果表明:在高要F0群体中29个微卫星位点共检测到242个等位基因,平均等位基因数 (Na) 为8.345,平均观测杂合度(Ho)为0.621,平均多态性信息 含量(PIC)为0.842,各位点PIC值均大于0.5,具高度遗传多样性;29个微卫星标记中有6个标记(GM114、GM213、GM423、GM642、UNH130 和UNH985)在高要F0群体中与至少一种生长性状显著相关(P<0.05);GM213、GM642、UNH130和UNH985 4个 标记的不同基因型个体对应的生长性状在高要F1群体中存在显著性差异(P<0.05),说明其在不同世代中遗传稳定;初筛生长相关微卫星标记在外群中的验证结果显示,GM213、GM642、UNH130 和UNH985 4个标记的不同基因型对应的生长性状在番禺群体中存在显著性差异(P<0.05),GM642、UNH130和UNH985 3个标记的不同基因型对应的生长性状在海南群体中存在显著性差异(P<0.05)。研究表明,GM642、UNH130和UNH985 3个微卫星位点在尼罗罗非鱼不同世代和不同群体中均与生长性状显著相关,GM642的DD和HH基因型,UNH130的AD、BC和FF基因型,以及UNH985的GH和CF基因型具有明显生长优势,可作为尼罗罗非鱼潜在的生长相关分子标记。
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出版历程
收稿日期:  2023-04-04

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