Screening of SNP locus and association with growth traits of MYF5 gene in Nile tilapia (Oreochromis niloticus)
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摘要: 为探寻肌源性调节因子5基因（MYF5）的单核苷酸多态性（SNP）标记与尼罗罗非鱼（Oerochromis niloticus）生长性状的相关性，采用PCR和测序法筛查SNP，并通过测序方法确认，从筛查到的35个SNP位点中挑选多态性较好的30个位点在尼罗罗非鱼高要亲代群体中进行分型分析，并与生长性状进行关联分析，将获得的生长相关位点在高要子代群体、番禺群体及海南群体中进行验证。结果表明：高要亲代群体中S18（G-1739C）位点与体质量、体宽显著相关（P<0.05），S3（A-113G）和S4（C-170T）位点与全长显著相关（P<0.05），S5（C-323T）和S24（C-2345T）位点与头长显著相关（P<0.05）；高要子代群体中，S3、S4位点与体质量显著相关（P<0.05），S24位点与体长、体高显著相关（P<0.05）；在番禺群体中获得的与体质量显著相关的SNP位点分别为S7（A-626T）、S21（C-2090G）和S32（G-2759T），与全长显著相关的SNP位点为S7；海南群体中未获得与生长性状显著相关的SNP位点；双倍型与各群体生长性状关联分析表明，在高要亲代群体中获得与全长、头长、体高显著相关的双倍型各1个，在高要子代群体中获得与体质量显著相关的双倍型1个，在番禺群体及海南群体中未获得与生长性状相关的双倍型。研究表明，从高要群体MYF5基因上获得的S3、S4和S18 3个SNP 位点可能影响尼罗罗非鱼的生长性状或与之紧密连锁，这些位点可以作为候选基因标记，用于尼罗罗非鱼生长相关分子标记辅助育种研究。Abstract: To explore the correlation between single nucleotide polymorphisms (SNP) markers of the MYF5 gene and the growth traits of Nile tilapia (Oreochromis niloticus), SNP loci were screened through PCR and sequencing, and confirmed through sequencing methods. Thirty SNP loci with high polymorphism were selected from 35 loci for genotyping analysis in the Gaoyao parent population, and association analysis between SNP loci and growth-related traits was conducted. The obtained growth related loci in Gaoyao broodstock population were verified in Gaoyao offspring population and Panyu populations. Finally, the growth traits related loci obtained above were subjected to genotyping analysis in the Hainan population for further validation. The results showed that S18 (G-1739C) was correlated with body weight and body width, S3 (A-113G) and S4 (C-170T) loci were correlated with total length, and S5 (C-323T) and S24 (C-2345T) were correlated with head length, in the Gaoyao broodstock population. In the Gaoyao offspring population, S3 and S4 loci were related to body weight; S24 was related to body length and body height. Three SNP loci related with body weight were obtained in the Panyu population, namely S7 (A-626T), S21 (C-2090G), and S32 (G-2759T), with one locus S7 related with total-length. No growth-trait related loci were found in the Hainan population. The analysis of the correlation between diplotype and growth traits in various populations showed that one diplotype significantly correlated with total length, one diplotype significantly correlated with head length, and one diplotype significantly correlated with body height was obtained in the Gaoyao broodstock population. One diplotype significantly correlated with body weight was obtained in the Gaoyao offspring population. No diploidy related to growth traits was found in the Panyu and Hainan populations.The findings suggest that S3, S4 and S18 SNP loci obtained from the MYF5 gene in this study might link or be closely related to the growth performance of Nile tilapia. These loci can be used as candidate gene markers for growth related molecular marker assisted breeding of Nile tilapia.
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Key words:
- Oreochromis niloticus /
- MYF5 /
- SNP /
- diploid /
- growth-related trait
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