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ISSN 2095-1388

主管 辽宁省教育厅

主办 大连海洋大学

尼罗罗非鱼MYF5基因SNP位点筛选及其与不同群体生长性状的关联分析

2023. 尼罗罗非鱼MYF5基因SNP位点筛选及其与不同群体生长性状的关联分析. 大连海洋大学学报, 38(6): 935-946. doi: 10.16535/j.cnki.dlhyxb.2023-073
引用本文: 2023. 尼罗罗非鱼MYF5基因SNP位点筛选及其与不同群体生长性状的关联分析. 大连海洋大学学报, 38(6): 935-946. doi: 10.16535/j.cnki.dlhyxb.2023-073
TONG Yannan, GAO Fengying, LIU Zhigang, WANG Miao, CAO Jianmeng, YI Mengmeng, KE Xiaoli, LU Maixin, ZHU Hai. 2023. Screening of SNP locus and association with growth traits of MYF5 gene in Nile tilapia (Oreochromis niloticus). Journal of Dalian Ocean University, 38(6): 935-946. doi: 10.16535/j.cnki.dlhyxb.2023-073
Citation: TONG Yannan, GAO Fengying, LIU Zhigang, WANG Miao, CAO Jianmeng, YI Mengmeng, KE Xiaoli, LU Maixin, ZHU Hai. 2023. Screening of SNP locus and association with growth traits of MYF5 gene in Nile tilapia (Oreochromis niloticus). Journal of Dalian Ocean University, 38(6): 935-946. doi: 10.16535/j.cnki.dlhyxb.2023-073

尼罗罗非鱼MYF5基因SNP位点筛选及其与不同群体生长性状的关联分析

  • 基金项目:

    海南省重点研发计划项目(ZDYF2020096);财政部和农业农村部:国家现代农业产业技术体系资助(CARS-46);2022年省级乡村振兴战略专项资金种业振兴项目(2022-SPY-00-005);广东省重点领域研发计划项目(2021B0202020001)

详细信息
    作者简介:

    佟延南(1984-),男,高级工程师。E-mail:444214983@qq.com

  • 中图分类号: S 917.4

Screening of SNP locus and association with growth traits of MYF5 gene in Nile tilapia (Oreochromis niloticus)

  • Fund Project: 海南省重点研发计划项目(ZDYF2020096);财政部和农业农村部:国家现代农业产业技术体系资助(CARS-46);2022年省级乡村振兴战略专项资金种业振兴项目(2022-SPY-00-005);广东省重点领域研发计划项目(2021B0202020001)
  • 为探寻肌源性调节因子5基因(MYF5)的单核苷酸多态性(SNP)标记与尼罗罗非鱼(Oerochromis niloticus)生长性状的相关性,采用PCR和测序法筛查SNP,并通过测序方法确认,从筛查到的35个SNP位点中挑选多态性较好的30个位点在尼罗罗非鱼高要亲代群体中进行分型分析,并与生长性状进行关联分析,将获得的生长相关位点在高要子代群体、番禺群体及海南群体中进行验证。结果表明:高要亲代群体中S18(G-1739C)位点与体质量、体宽显著相关(P<0.05),S3(A-113G)和S4(C-170T)位点与全长显著相关(P<0.05),S5(C-323T)和S24(C-2345T)位点与头长显著相关(P<0.05);高要子代群体中,S3、S4位点与体质量显著相关(P<0.05),S24位点与体长、体高显著相关(P<0.05);在番禺群体中获得的与体质量显著相关的SNP位点分别为S7(A-626T)、S21(C-2090G)和S32(G-2759T),与全长显著相关的SNP位点为S7;海南群体中未获得与生长性状显著相关的SNP位点;双倍型与各群体生长性状关联分析表明,在高要亲代群体中获得与全长、头长、体高显著相关的双倍型各1个,在高要子代群体中获得与体质量显著相关的双倍型1个,在番禺群体及海南群体中未获得与生长性状相关的双倍型。研究表明,从高要群体MYF5基因上获得的S3、S4和S18 3个SNP 位点可能影响尼罗罗非鱼的生长性状或与之紧密连锁,这些位点可以作为候选基因标记,用于尼罗罗非鱼生长相关分子标记辅助育种研究。
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  • OLSON E N.MyoD family:a paradigm for development?[J].Genes & Development, 1990, 4(9):1454-1461.

    HUGHES S M, CHI M M, LOWRYO H, et al.Myogenin induces a shift of enzyme activity from glycolytic to oxidative metabolism in muscles of transgenic mice[J].The Journal of Cell Biology, 1999, 145(3):633-642.

    OTT M O, BOBER E, LYONS G, et al.Early expression of the myogenic regulatory gene, Myf-5, in precursor cells of skeletal muscle in the mouse embryo[J].Development, 1991, 111(4):1097-1107.

    TIMMONS J A, WENNMALM K, LARSSON O, et al.Myogenic gene expression signature establishes that brown and white adipocytes originate from distinct cell lineages[J].Proceedings of the National Academy of Sciences of the United States of America, 2007, 104(11):4401-4406.

    HOU G Y, ZHOU H L, CAO T, et al.Expression and variation of Myf5 and MyoD1 genes in different tissues of Wuzhishan pigs[J].Genetics and Molecular Research, 2015, 14(2):3729-3735.

    YAMAMOTO M, LEGENDREN P, BISWAS A A, et al.Loss of MyoD and Myf5 in skeletal muscle stem cells results in altered myogenic programming and failed regeneration[J].Stem Cell Reports, 2018, 10(3):956-969.

    ZHAO C P, RAZA S H A, KHAN R, et al.Genetic variants in MYF5 affected growth traits and beef quality traits in Chinese Qinchuan cattle[J].Genomics, 2020, 112(4):2804-2812.

    ZHANG Z C, LIU C, HAO W J, et al.Novelsingle nucleotide polymorphisms and haplotype of MYF5 gene are associated with body measurements and ultrasound traits in grassland short-tailed sheep[J].Genes, 2022, 13(3):483.

    TANG Y, ZHANG T, ZHANG G X, et al.Eight SNPs of the Myf5 gene and diplotypes associated with growth and reproductive traits in Jinghai yellow chicken[J].Molecular Biology Reports, 2014, 41(10):6837-6844.

    WANG J, HU Y S, ELZO M A, et al.Genetic effect of Myf5 gene in rabbit meat quality traits[J].Journal of Genetics, 2017, 96(4):673-679.

    GUO Y H, BAI J J, LAO H H, et al.Cloning and analysis of Myf5 cDNA and genomic sequence of largemouth bass (Micropterus salmoides)[J].Journal of Fishery Sciences of China, 2008, 15(4):568-576.(in Chinese)

    GUO Y H, BAI J J, CHANG O Q, et al.Molecular structure of the largemouth bass (Micropterus salmoides) Myf5 gene and its effect on skeletal muscle growth[J].Molecular Biology Reports, 2009, 36(6):1497-1504.

    ZHONG M C, ZHENG G M, ZHAO J, et al.Cloning and SNPs analysis of Myf5 gene from Cirrhinus molitorella[J].Journal of Fishery Sciences of China, 2010, 17(4):681-688.(in Chinese)

    CHEN B L, XIAO W, ZOU Z Y, et al.Ghrelin gene single nucleotide polymorphisms and their effects on Nile tilapia (Oreochromis niloticus) growth[J].Aquaculture Reports, 2020, 18:100469.

    LIU H, BAI J J, LI S J, et al.SNPs detection of ghrelin gene and its association with growth traits in largemouth bass(Micropterus salmoides)[J].Journal of Fisheries of China, 2016, 40(4):521-527.(in Chinese)

    LIU H, BAI J J, LI S J, et al.SNPs detection of PACAP gene and its association with growth traits in largemouth bass(Micropterus salmoides)[J].Journal of Agricultural Biotechnology, 2016, 24(3):390-396.(in Chinese)

    WANG C X, LU M X, GAO F Y, et al.Screening of single nucleotide polymophisms(SNPs) related with growth in growth hormone secretagogue receptor gene(GHSR) of Nile tilapia(Oreochromis niloticus)[J].Journal of Agricultural Biotechnology, 2015, 23(6):762-771.(in Chinese)

    WANG C X, LU M X, GAO F Y, et al.The polymorphism of ghrelin gene of Oreochromis niloticus and identification of its SNP loci associated with the growth traits[J].Acta Hydrobiologica Sinica, 2016, 40(1):50-57.(in Chinese)

    CHEN B L, XIAO W, ZOU Z Y, et al.Correlation analysis of polymorphisms in promoter region and coding region of GHR and IGF-Ⅰ genes with growth traits of two varieties of Nile tilapia(Oreochromis niloticus)[J].Journal of Agricultural Biotechnology, 2020, 28(11):2032-2047.(in Chinese)

    CHEN X F, YANG G L, YU J H, et al.Isolation of IGF2 gene and correlation of its SNPs with fish sharp and weight gain in GIFT strain Nile tilapia Oreochromis niloticus[J].Chinese Journal of Zoology, 2010, 45(2):107-114.(in Chinese)

    HALUSHKA M K, FAN J B, BENTLEY K, et al.Patterns of single-nucleotide polymorphisms in candidate genes for blood-pressure homeostasis[J].Nature Genetics, 1999, 22(3):239-247.

    RAFALSKI J A.Novel genetic mapping tools in plants:SNPs and LD-based approaches[J].Plant Science, 2002, 162(3):329-333.

    NIE Q H, LEI M M, OUYANG J H, et al.Identification and characterization of single nucleotide polymorphisms in 12 chicken growth-correlated genes by denaturing high performance liquid chromatography[J].Genetics, Selection, Evolution, 2005, 37(3):339-360.

    JING Y J, BAI J J, LI S J, et al.Analysis of EST database for two subspecies of largemouth bass[J].Journal of Shanghai Ocean University, 2012, 21(6):945-950.(in Chinese)

    ZHU B, FAN J J, BAI J J, et al.Gold grass carp microsatellite polymorphism and its comparative analysis with four grass carp populations from China[J].South China Fisheries Science, 2017, 13(2):51-58(in Chinese)

    CHEN B L, XIAO W, ZOU Z Y, et al.The effects of single nucleotide polymorphisms in Neuropeptide Y and prepro-orexin on growth in Nile tilapia (Oreochromis niloticus)[J].Aquaculture, 2021, 543:736974.

    XU L, BAI J J, LI S J, et al.Pyramiding of growth-related genotypes in generations of the largemouth bass, Micropterus salmoides, ‘Youlu No.1’[J].Journal of South China Agricultural University, 2014, 35(1):7-11.(in Chinese)

    MA D M, HAN L Q, BAI J J, et al.A 66-bp deletion in growth hormone releasing hormone gene 5'-flanking region with largemouth bass recessive embryonic lethal[J].Animal Genetics, 2014, 45(3):421-426.

    DUAN J B, WAINWRIGHT M S, COMERON J M, et al.Synonymous mutations in the human dopamine receptor D2(DRD2) affect mRNA stability and synthesis of the receptor[J].Human Molecular Genetics, 2003, 12(3):205-216.

    KIMCHI-SARFATY C, OH J M, KIM I W, et al.A "silent" polymorphism in the MDR1 gene changes substrate specificity[J].Science, 2007, 315(5811):525-528.

    SAPRA A K, ARAVA Y, KHANDELIA P, et al.Genome-wide analysis of pre-mRNA splicing:intron features govern the requirement for the second-step factor, Prp17 in Saccharomyces cerevisiae and Schizosaccharomyces pombe[J].The Journal of Biological Chemistry, 2004, 279(50):52437-52446.

    NOTT A, MEISLIN S H, MOORE M J.A quantitative analysis of intron effects on mammalian gene expression[J].RNA, 2003, 9(5):607-617.

    TAN X, TONG J G.SNPs and their applications in studies on genetics and breeding of aquaculture animals[J].Acta Hydrobiologica Sinica, 2011, 35(2):348-354.(in Chinese)

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出版历程
收稿日期:  2023-04-03

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