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ISSN 2095-1388

主管 辽宁省教育厅

主办 大连海洋大学

敲除hdac8基因对斑马鱼低温耐受能力的影响

史雪灵 罗军涛 罗贝贝 韩兵社 张俊芳

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史雪灵, 罗军涛, 罗贝贝, 韩兵社, 张俊芳. 2023. 敲除hdac8基因对斑马鱼低温耐受能力的影响. 大连海洋大学学报, 38(6): 964-971. doi: 10.16535/j.cnki.dlhyxb.2023-061
引用本文: 史雪灵, 罗军涛, 罗贝贝, 韩兵社, 张俊芳. 2023. 敲除hdac8基因对斑马鱼低温耐受能力的影响. 大连海洋大学学报, 38(6): 964-971. doi: 10.16535/j.cnki.dlhyxb.2023-061
SHI Xueling, LUO Juntao, LUO Beibei, HAN Bingshe, ZHANG Junfang. 2023. Effects of hdac8 knockout on low temperature tolerance in zebrafish (Danio rerio). Journal of Dalian Ocean University, 38(6): 964-971. doi: 10.16535/j.cnki.dlhyxb.2023-061
Citation: SHI Xueling, LUO Juntao, LUO Beibei, HAN Bingshe, ZHANG Junfang. 2023. Effects of hdac8 knockout on low temperature tolerance in zebrafish (Danio rerio). Journal of Dalian Ocean University, 38(6): 964-971. doi: 10.16535/j.cnki.dlhyxb.2023-061

敲除hdac8基因对斑马鱼低温耐受能力的影响

  • 基金项目:

    上海市科技兴农重点攻关项目[沪农科创字(2019)第1-2号];国家自然科学基金面上项目(81770165);上海市教委水产一流学科建设项目

详细信息
    作者简介:

    史雪灵(1998-),女,硕士研究生。E-mail:215259514@qq.com

  • 中图分类号: S 965.9

Effects of hdac8 knockout on low temperature tolerance in zebrafish (Danio rerio)

  • Fund Project: 上海市科技兴农重点攻关项目[沪农科创字(2019)第1-2号];国家自然科学基金面上项目(81770165);上海市教委水产一流学科建设项目
  • 为探究敲除组蛋白去乙酰化酶8基因 (histone deacetylase 8, hdac8) 对斑马鱼(Danio rerio)低温耐受能力的影响,采用组织学、生理和生化等方法,研究了低温(8 ℃) 短期胁迫下hdac8基因敲除(hdac8-/-)斑马鱼和野生型(WT)斑马鱼的组织损伤、氧化应激相关指标及其凋亡相关基因表达的变化。结果表明:低温胁迫下,hdac8-/-斑马鱼的半数致死时间(LT50)明显缩短,hdac8-/-斑马鱼的LT50为14.5 h,WT斑马鱼的LT50为21.5 h;HE染色显示,低温胁迫下,hdac8-/-斑马鱼鳃丝、肝脏和骨骼肌相较于WT斑马鱼损伤更加严重;28 ℃下,WT和hdac8-/-斑马鱼的活性氧(ROS)、丙二醛(MDA)水平无显著性变化(P>0.05),而hdac8-/-斑马鱼的ATP水平则显著降低(P<0.05);低温胁迫下,hdac8-/-斑马鱼的 ROS和MDA水平显著高于WT斑马鱼(P<0.05),而ATP水平则显著低于WT斑马鱼(P<0.05);RT-qPCR显示,28 ℃下, hdac8-/-斑马鱼caspase3基因表达显著上调(P<0.05),其他抗氧化与凋亡相关基因无显著性变化(P>0.05);低温胁迫下,WT和hdac8-/-斑马鱼sodcatcaspase3、caspase9和bax基因表达水平显著上调(P<0.05),且hdac8-/-斑马鱼的这5种基因表达水平均显著高于WT斑马鱼(P<0.05)。研究表明,敲除hdac8基因显著降低了斑马鱼的低温耐受能力,并促进了低温氧化应激损伤和细胞凋亡。
  • 加载中
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收稿日期:  2023-03-26

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