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ISSN 2095-1388

主管 辽宁省教育厅

主办 大连海洋大学

敲除hdac8基因对斑马鱼低温耐受能力的影响

2023. 敲除hdac8基因对斑马鱼低温耐受能力的影响. 大连海洋大学学报, 38(6): 964-971. doi: 10.16535/j.cnki.dlhyxb.2023-061
引用本文: 2023. 敲除hdac8基因对斑马鱼低温耐受能力的影响. 大连海洋大学学报, 38(6): 964-971. doi: 10.16535/j.cnki.dlhyxb.2023-061
SHI Xueling, LUO Juntao, LUO Beibei, HAN Bingshe, ZHANG Junfang. 2023. Effects of hdac8 knockout on low temperature tolerance in zebrafish (Danio rerio). Journal of Dalian Ocean University, 38(6): 964-971. doi: 10.16535/j.cnki.dlhyxb.2023-061
Citation: SHI Xueling, LUO Juntao, LUO Beibei, HAN Bingshe, ZHANG Junfang. 2023. Effects of hdac8 knockout on low temperature tolerance in zebrafish (Danio rerio). Journal of Dalian Ocean University, 38(6): 964-971. doi: 10.16535/j.cnki.dlhyxb.2023-061

敲除hdac8基因对斑马鱼低温耐受能力的影响

  • 基金项目:

    上海市科技兴农重点攻关项目[沪农科创字(2019)第1-2号];国家自然科学基金面上项目(81770165);上海市教委水产一流学科建设项目

详细信息
    作者简介:

    史雪灵(1998-),女,硕士研究生。E-mail:215259514@qq.com

  • 中图分类号: S 965.9

Effects of hdac8 knockout on low temperature tolerance in zebrafish (Danio rerio)

  • Fund Project: 上海市科技兴农重点攻关项目[沪农科创字(2019)第1-2号];国家自然科学基金面上项目(81770165);上海市教委水产一流学科建设项目
  • 为探究敲除组蛋白去乙酰化酶8基因 (histone deacetylase 8, hdac8) 对斑马鱼(Danio rerio)低温耐受能力的影响,采用组织学、生理和生化等方法,研究了低温(8 ℃) 短期胁迫下hdac8基因敲除(hdac8-/-)斑马鱼和野生型(WT)斑马鱼的组织损伤、氧化应激相关指标及其凋亡相关基因表达的变化。结果表明:低温胁迫下,hdac8-/-斑马鱼的半数致死时间(LT50)明显缩短,hdac8-/-斑马鱼的LT50为14.5 h,WT斑马鱼的LT50为21.5 h;HE染色显示,低温胁迫下,hdac8-/-斑马鱼鳃丝、肝脏和骨骼肌相较于WT斑马鱼损伤更加严重;28 ℃下,WT和hdac8-/-斑马鱼的活性氧(ROS)、丙二醛(MDA)水平无显著性变化(P>0.05),而hdac8-/-斑马鱼的ATP水平则显著降低(P<0.05);低温胁迫下,hdac8-/-斑马鱼的 ROS和MDA水平显著高于WT斑马鱼(P<0.05),而ATP水平则显著低于WT斑马鱼(P<0.05);RT-qPCR显示,28 ℃下, hdac8-/-斑马鱼caspase3基因表达显著上调(P<0.05),其他抗氧化与凋亡相关基因无显著性变化(P>0.05);低温胁迫下,WT和hdac8-/-斑马鱼sodcatcaspase3、caspase9和bax基因表达水平显著上调(P<0.05),且hdac8-/-斑马鱼的这5种基因表达水平均显著高于WT斑马鱼(P<0.05)。研究表明,敲除hdac8基因显著降低了斑马鱼的低温耐受能力,并促进了低温氧化应激损伤和细胞凋亡。
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  • WANG H M, WANG Y, NIU M H, et al.Cold acclimation for enhancing the cold tolerance of zebrafish cells[J].Frontiers in Physiology, 2022, 12:813451.

    WILHELM FILHO D.Reactive oxygen species, antioxidants and fish mitochondria[J].Frontiers in Bioscience, 2007, 12(1):1229-1237.

    SONG H, XU D, TIAN L, et al.Overwinter mortality in yellow drum (Nibea albiflora):insights from growth and immune responses to cold and starvation stress[J].Fish & Shellfish Immunology, 2019, 92:341-347.

    YAN Q Q, LI W H, GONG X T, et al.Transcriptomic and phenotypic analysis of CRISPR/Cas9-mediated gluk2 knockout in zebrafish[J].Genes, 2022, 13(8):1441.

    CHEN K, LI X, SONG G, et al.Deficiency in the membrane protein Tmbim3a/Grinaa initiates cold-induced ER stress and cell death by activating an intrinsic apoptotic pathway in zebrafish[J].Journal of Biological Chemistry, 2019, 294(30):11445-11457.

    CHENG C H, GUO Z X, WANG A L.The protective effects of taurine on oxidative stress, cytoplasmic free-Ca2+ and apoptosis of pufferfish (Takifugu obscurus) under low temperature stress[J].Fish & Shellfish Immunology, 2018, 77:457-464.

    ASADI M, TAGHIZADEH S, KAVIANI E, et al.Caspase-3:structure, function, and biotechnological aspects[J].Biotechnology and Applied Biochemistry, 2022, 69(4):1633-1645.

    HU L H, WANG Y, WANG H M, et al.Effects of different temperature stress on gill apoptosis of medaka Oryzias latipes[J].Journal of Dalian Ocean University, 2021, 36(6):929-936.(in Chinese)

    LI Y, HUANG J S, CHEN Y M, et al.Effects of low-temperature stress on serum biochemical, antioxidant enzymes activities and apoptosis-related gene expression in liver of juvenile cobia(Rachycentron canadum)[J].Journal of Guangdong Ocean University, 2022, 42(5):18-26.(in Chinese)

    SUN Z, TAN X, XU M, et al.Liver transcriptome analysis and de novo annotation of the orange-spotted groupers (Epinephelus coioides) under cold stress[J].Comparative Biochemistry and Physiology Part D:Genomics and Proteomics, 2019, 29:264-273.

    KIM J Y, CHO H, YOO J, et al.Pathological role of HDAC8:cancer and beyond[J].Cells, 2022, 11(19):3161.

    YAO Y L, HAO F, TANG L C, et al.Downregulation of HDAC8 expression decreases CD163 levels and promotes the apoptosis of macrophages by activating the ERK signaling pathway in recurrent spontaneous miscarriage[J].Molecular Human Reproduction, 2020, 26(7):521-531.

    KANG Y, NIAN H, RAJENDRAN P, et al.HDAC8 and STAT3 repress BMF gene activity in colon cancer cells[J].Cell Death & Disease, 2014, 5(10):e1476.

    BALASUBRAMANIAN S, RAMOS J, LUO W, et al.A novel histone deacetylase 8(HDAC8)-specific inhibitor PCI-34051 induces apoptosis in T-cell lymphomas[J].Leukemia, 2008, 22(5):1026-1034.

    TIAN Y, WONG V W, WONG G L, et al.Histone deacetylase HDAC8 promotes insulin resistance and β-catenin activation in NAFLD-associated hepatocellular carcinoma[J].Cancer Research, 2015, 75(22):4803-4816.

    LUO B B, LUO J T, HAN L J, et al.Effects of hdac8 gene knockout on locomotion capacity of zebrafish[J].Journal of Biology, 2023, 40(3):74-79.(in Chinese)

    HU P, LIU M L, LIU Y M, et al.Transcriptome comparison reveals a genetic network regulating the lower temperature limit in fish[J].Scientific Reports, 2016, 6:28952.

    WU S M, LIU J H, SHU L H, et al.Anti-oxidative responses of zebrafish (Danio rerio) gill, liver and brain tissues upon acute cold shock[J].Comparative Biochemistry and Physiology Part A:Molecular & Integrative Physiology, 2015, 187:202-213.

    QIU J, WANG W N, WANG L J, et al.Oxidative stress, DNA damage and osmolality in the Pacific white shrimp, Litopenaeus vannamei exposed to acute low temperature stress[J].Comparative Biochemistry and Physiology Part C:Toxicology & Pharmacology, 2011, 154(1):36-41.

    PANG X, FU S J, ZHANG Y G.Acclimation temperature alters the relationship between growth and swimming performance among juvenile common carp (Cyprinus carpio)[J].Comparative Biochemistry and Physiology Part A:Molecular & Integrative Physiology, 2016, 199:111-119.

    TAO Y F, QIANG J, WANG H, et al.Acute toxicity of low-pH stress and its effect on enzyme activity and histological structure of gill and hepatopancreas in Procambarus clarkii[J].Journal of Fishery Sciences of China, 2016, 23(6):1279-1289.(in Chinese)

    YU Z X, REN X Y, SHAO H X, et al.Effect of low temperature stress on antioxidant system and apoptosis of Marsupenaeus japonicus[J].Progress in Fishery Sciences, 2022, 43(2):157-166.(in Chinese)

    (6):14-17.(in Chinese)

    XU W J, LI H Y, WU L Y, et al.Genetically based physiological responses to overwinter starvation in gibel carp (Carassius gibelio)[J].Frontiers in Endocrinology, 2020, 11:578777.

    KRATZ E, EIMON P M, MUKHYALA K, et al.Functional characterization of the Bcl-2 gene family in the zebrafish[J].Cell Death & Differentiation, 2006, 13(10):1631-1640.

    WANG Y, WANG H M, HU L H, et al.Leptin gene protects against cold stress in Antarctic toothfish[J].Frontiers in Physiology, 2021, 12:740806.

    LU X Q, ZHANG L L, HUANG S Y, et al.Cloning and expression analysis of caspase-3 gene in development, immune and stress of variously colored abalone Haliotis diversicolor[J].Journal of Dalian Ocean University, 2022, 37(3):411-419.(in Chinese)

    KALPAGE H A, BAZYLIANSKA V, RECANATI M A, et al.Tissue-specific regulation of cytochrome c by post-translational modifications:respiration, the mitochondrial membrane potential, ROS, and apoptosis[J].The FASEB Journal, 2019, 33(2):1540-1553.

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出版历程
收稿日期:  2023-03-26

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