Effects of iced trash fish and formulated diet on growth performance, intestinal structure and microflora diversity of marbled flounder (Pseudopleuronectes yokohamae)
-
摘要: 为研究冰鲜杂鱼和配合饲料对钝吻黄盖鲽(Pseudopleuronectes yokohamae)生长及肠道健康的影响,以同一批培育的钝吻黄盖鲽幼鱼(体长为10.16 cm±0.06 cm)为研究对象,经42 d的投喂试验,采用体尺性状测量、肠道组织石蜡切片制作和高通量测序等方法,研究了冰鲜杂鱼(FF组)、配合饲料(CF组)及二者混合投喂(MF组)对钝吻黄盖鲽生长性能、肠道结构和肠道菌群的影响。结果表明:与配合饲料组 相比,冰鲜杂鱼组幼鱼的增重率、特定生长率显著提高(P<0.05), 饲料系数显著降低(P<0.05); 幼鱼肠道及其菌群结构分析显示,投喂冰鲜杂鱼能显著提高幼鱼肠道绒毛长度和基层厚度(P<0.05),提高幼鱼肠道菌群多样性,并促进红杆菌科等有益菌在肠道中的分布;混合投喂组各项指标介于冰鲜杂鱼组与配合饲料组之间。研究表明,在钝吻黄盖鲽的养殖过程中,可在配合饲料中适量添加红杆菌科的有益菌以促进钝吻黄盖鲽的生长和营养吸收, 而钝吻黄盖鲽人工配合饲料的配方还应进一步优化,以提高其市场优势。Abstract: In order to evaluate effects of iced trash fish, formulated diet and their mixture on growth, intestinal structure and microflora of marbled flounder (Pseudopleuronectes yokohamae), the same batch of marbled flounder juveniles with body length of (10.16±0.06)cm were reared in a 126 L plastic tank and fed iced trash fish, formulated diet and their mixture at water temperature of (15±1)℃ for 42 days. The results showed that the fish fed iced trash had significant increase in the body weight gain and specific growth rate (P<0.05), and decrease in the feed conversion ratio (P<0.05). The intestinal histological structure showed that the length of intestinal villi and the thickness of basal layer were significantly increased in the fish fed iced trash (P<0.05). The intestinal microflora analysis showed that the fish fed iced trash had increase in the intestinal flora diversity, promoting the distribution of beneficial bacteria such as Rhodobacteraceae in the intestinal tract of marbled flounder. It was speculated that the marbled flounder fed formulated diet had slower growth may be involved in the poorly developed intestinal mucosa and the decline in the ratio of Bacteroides to Firmicutes. The indices in the mixed diet group were mediated between iced trash fish group and formulated feed group. Therefore, beneficial bacteria in Rhodobacteraceae were added in diet to promote the growth and nutrient absorption of marbled flounder during cultivation. The artificial compound feed of marbled flounder should be further optimized to improve market advantage.
-
-
-216.(in Chinese)
ZHANG Y,XIAO Y S,GAO T X,et al.Analysis of genetic diversity of natural population in Pleuronectes yokohamae[J].Journal of Fisheries of China,2008,32(3):492-496.(in Chinese)
DOU S Z,YANG J M.Seasonal changes of feeding habits and food intake of Pseudopleuronectes yokohamae in the Yellow River Mouth[J].Haiyang Xuebao,1992,14(6):103-112.(in Chinese)
WANG Y H,DING W,CHEN J,et al.Effects of mixed feed and chilled mixed fish on growth rate and feed cost of Scophthalmus maximus[J].Jiangsu Agricultural Sciences,2016,44(7):282-285.(in Chinese)
LI Z F,GONG W B,WANG J L,et al.Evaluation of effects of frozen fresh fish and artificial compound feed on muscle quality and health status of Micropterus salmoides[J].Chinese Journal of Animal Nutrition,2017,29(11):4180-4188.(in Chinese)
GUAN S J,WU R Q,XIE J,et al.Effects of two diets on growth, digestive tract index and digestive enzyme activities of Micropterus salmoides[J].Feed Industry,2007,28(2):32-36.(in Chinese)
YU E M,ZHANG Z N,XIA Y,et al.Effects of different diets on intestinal microflora of largemouth bass (Micropterus salmoides)[J].Journal of Fisheries of China,2015,39(1):118-126.(in Chinese)
INGERSLEV H C,JØRGENSEN L V G,STRUBE M L,et al.The development of the gut microbiota in rainbow trout (Oncorhynchus mykiss) is affected by first feeding and diet type[J].Aquaculture,2014,424/425:24-34.
DUAN J C,HU J H,SHEN Y H,et al.Effect of Enterocytozoon hepatopenaei infection on the intestinal microflora of Exopalaemon carinicauda[J].Progress in Fishery Sciences,2022,43(3):75-83.(in Chinese)
WANG Q,GARRITY G M,TIEDJE J M,et al.Naive Bayesian classifier for rapid assignment of rRNA sequences into the new bacterial taxonomy[J].Applied and Environmental Microbiology,2007,73(16):5261-5267.
TONG W,ZHANG J S,KOU F,et al.A comparative study on Scophthalmus maximus by using full price combined bait and chilled mixed fish[J].Hebei Fisheries,2014(2):38-39,69.(in Chinese)
CONG L M,WANG W F,GAO C R,et al.Effects of compound diet and fresh frozen Ammodytes personatus on growth,antioxidant ability and lipid metabolism of hybrid grouper(Epinephelus fuscoguttatus ♀×Epinephelus lanceolatus♂) juveniles[J].Journal of Fisheries of China,2016,40(9):1398-1407.(in Chinese)
ZHU Z M,MA D M,BAI J J,et al.Effects of formulated diets and frozen trash fish on growth and expression of LPL gene mRNA in largemouth bass Micropterus salmoides[J].Journal of Dalian Ocean University,2014,29(4):360-363.(in Chinese)
WANG Y P,LU Y,ZHANG Y,et al.The draft genome of the grass carp (Ctenopharyngodon idellus) provides insights into its evolution and vegetarian adaptation[J].Nature Genetics,2015,47(6):625-631.
SHU Y L,HONG P,TANG D,et al.Comparison of intestinal microbes in female and male Chinese concave-eared frogs (Odorrana tormota) and effect of nematode infection on gut bacterial communities[J].Microbiology Open,2019,8(6):e00749.
MANSFIELD G S,DESAI A R,NILSON S A,et al.Characterization of rainbow trout (Oncorhynchus mykiss) intestinal microbiota and inflammatory marker gene expression in a recirculating aquaculture system[J].Aquaculture,2010,307(1/2):95-104.
SHIN N R,WHON T W,BAE J W.Proteobacteria:microbial signature of dysbiosis in gut microbiota[J].Trends in Biotechnology,2015,33(9):496-503.
DU R,WANG M G,PENG J J,et al.Advances in discovery of new antibiotics in uncultured microorganisms[J].Chinese Journal of Bioprocess Engineering,2022,20(2):172-181.(in Chinese)
EVANS N J,BROWN J M,MURRAY R D,et al.Characterization of novel bovine gastrointestinal tract Treponema isolates and comparison with bovine digital dermatitis treponemes[J].Applied and Environmental Microbiology,2011,77(1):138-147.
WU F,WEN H,JIANG M,et al.Effects of dietary vitamin B12 on growth,body composition and hemopoiesis of juvenile grass carp(Ctenopharyngodon idellus)[J].Journal of Jilin Agricultural University,2007,29(6):695-699.(in Chinese)
SONNENSCHEIN E C,NIELSEN K F,D'ALVISE P,et al.Global occurrence and heterogeneity of the Roseobacter clade species Ruegeria mobilis[J].The ISME Journal,2017,11(2):569-583.
计量
- 文章访问数: 657
- PDF下载数: 0
- 施引文献: 0
下载:
