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ISSN 2095-1388

主管 辽宁省教育厅

主办 大连海洋大学

尼罗罗非鱼跨膜Bax抑制剂母体6基因Tmbim 6的克隆、表达及功能鉴定

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2023. 尼罗罗非鱼跨膜Bax抑制剂母体6基因Tmbim 6的克隆、表达及功能鉴定. 大连海洋大学学报, 38(2): 275-283. doi: 10.16535/j.cnki.dlhyxb.2022-191
引用本文: 2023. 尼罗罗非鱼跨膜Bax抑制剂母体6基因Tmbim 6的克隆、表达及功能鉴定. 大连海洋大学学报, 38(2): 275-283. doi: 10.16535/j.cnki.dlhyxb.2022-191
WU Yiqin, CHEN Funuan, ZHANG Zhiqiang, JIANG Baijian, JIAN Jichang, HUANG Yu. 2023. Molecular cloning, expression and functions of Tmbim 6 gene in Nile tilapia (Oreochromis niloticus). Journal of Dalian Ocean University, 38(2): 275-283. doi: 10.16535/j.cnki.dlhyxb.2022-191
Citation: WU Yiqin, CHEN Funuan, ZHANG Zhiqiang, JIANG Baijian, JIAN Jichang, HUANG Yu. 2023. Molecular cloning, expression and functions of Tmbim 6 gene in Nile tilapia (Oreochromis niloticus). Journal of Dalian Ocean University, 38(2): 275-283. doi: 10.16535/j.cnki.dlhyxb.2022-191

尼罗罗非鱼跨膜Bax抑制剂母体6基因Tmbim 6的克隆、表达及功能鉴定

  • 基金项目:

    国家自然科学基金(32002426, 32073006)

    湛江市非资助科技攻关计划项目(2020B01169)

详细信息
    作者简介:

    巫祎琴(1998-),女,硕士研究生。E-mail:1525287075@qq.com

  • 中图分类号: S917.4

Molecular cloning, expression and functions of Tmbim 6 gene in Nile tilapia (Oreochromis niloticus)

  • Fund Project: 湛江市非资助科技攻关计划项目(2020B01169)
  • 为了解跨膜Bax抑制剂母体6(transmembrane Bax inhibitor motif containing 6, TMBIM 6)在尼罗罗非鱼(Oreochromis niloticus)病原体感染中的作用,利用聚合酶链式反应(PCR)对Tmbim 6基因进行克隆与鉴定,并采用实时荧光定量PCR(qPCR)分析Tmbim 6在健康尼罗罗非鱼(体质量为80~100 g)的组织分布模式及无乳链球菌(Streptococcus agalactiae)、Poly I:C模拟病毒刺激后的表达变化。结果表明:尼罗罗非鱼Tmbim 6开放阅读框为714 bp,可编码237个氨基酸,包含6个跨膜结构域和1个C-末端基序;亚细胞定位显示,尼罗罗非鱼TMBIM 6定位于细胞质,双荧光素酶报告基因系统检测发现,Tmbim 6在HEK-293T细胞中过表达后极显著抑制NF-κB通路(P<0.01);qPCR分析显示,Tmbim 6基因在所检测的组织中广泛分布,在肝脏和肌肉中的表达量最高;经无乳链球菌感染和Poly I:C刺激后,Tmbim 6在肝脏、脾脏、头肾、脑和肠道中的表达水平均显著上调(P<0.05)。研究表明,尼罗罗非鱼TMBIM 6参与了无乳链球菌感染和Poly I:C刺激后的细胞应激过程及免疫反应。
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收稿日期:  2022-06-14

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