Effects of MS-222 on anaesthesia, tissue structure and antioxidant enzyme activity of juvenile large yellow croaker (Larimichthys crocea)
-
摘要: 为探究MS-222长时间麻醉对大黄鱼(Larimichthys crocea)幼鱼机体的影响,采用不同质量浓度(10、20、30、40、50、60、70 mg/L)的MS-222,对体质量为(97.97±9.56)g的大黄鱼幼鱼进行24 h麻醉试验,通过对肝脏、鳃丝和脑的组织形态及超微结构变化进行显微和电镜观察,评估长时间麻醉对幼鱼机体的影响;通过对肝脏抗氧化酶活性和血清皮质醇浓度的检测,分析麻醉对机体抗逆性的影响。结果表明:在(16±1)℃条件下,用质量浓度为40 mg/L的MS-222麻醉时,幼鱼可进入深度镇静期,用60～70 mg/L的MS-222麻醉时,幼鱼可进入深度麻醉期,但用70 mg/L的MS-222麻醉24 h后,幼鱼的复苏率仅为50%;组织切片观察显示,用40 mg/L的MS-222麻醉24 h时,对机体可能存在轻微损伤;生理生化试验显示,用20 mg/L的MS-222麻醉时,幼鱼血清皮质醇浓度在1.5 h时显著上升(P<0.05),用20、40 mg/L的MS-222麻醉时,总体上能增强幼鱼肝脏中的超氧化物歧化酶(SOD)、过氧化氢酶(CAT)和谷胱甘肽过氧化物酶(GSH-Px)活力,在清水中复苏6 h后,SOD、CAT、GSH-Px活力基本能恢复至空白组水平。研究表明,40 mg/L的MS-222可作为本试验条件下大黄鱼幼鱼长时间运输的适宜质量浓度。Abstract: To explore the effects of long period of MS-222 anesthesia on juvenile large yellow croaker (Larimichthys crocea), juvenile large yellow croaker with body weight of (97.97±9.56) g were exposed to different MS-222 concentrations of 10, 20, 30, 40, 50, 60 and 70 mg/L in glass fiber reinforced plastic bucket at water temperature of (16±1)℃ for 24 h. Histological and electron microscopic structures were observed in the liver, and gill filament and antioxidant enzyme (superoxide dismutase SOD, catalase CAT, and glutathione oxidase GSH-Px) activities in live and serum cortisol level in the anesthetic large yellow croaker exposed to the different concentrations of MS-222. The results showed that the juveniles turned into the deep sedation period in anesthesia process at MS-222 concentration of 40 mg/L and into the deep anesthesia period at the concentration from 60 mg/L to 70 mg/L. However, the large yellow croaker juveniles had recovery rate of only 50% 24 hours after 70 mg/L anesthesia. The histological section and electron microscope observation showed that liver, gill and pituitary were slightly damaged by 40 mg/L anesthesia for 24 h. The antioxidant enzyme activity and serum cortisol level were found to be significantly increased at 20 mg/L MS-222 at 1.5 h (P<0.05), with an overall enhancement effect on activities of SOD, CAT, and GSH-Px at 20 and 40 mg/L MS-222 anesthesia. There was basic similarity in activities of SOD, CAT and GSH-Px between group 20 mg/L and group 40 mg/L 6 h after recovery in clean water. To sum up, according to the determination of anesthetic concentration of MS-222 on juvenile large yellow croaker and the study on MS-222 influence on the body, it is speculated that the concentration of 40 mg/L is the basis for long-term transportation.
-
Key words:
- Larimichthys crocea /
- MS-222 /
- histological structure /
- antioxidant enzyme activity
-
-
HONG W S,LIU J F,ZHENG W Q,et al.Transformation and upgrading countermeasure for the large yellow croaker industry in China[J].Journal of Fisheries Research,2018,40(4):315-323.(in Chinese)
ZHAO Y L,YANG X L,HUANG Y P,et al.Study on anesthesia effect of eugenol on large yellow croaker (Larimichthys crocea)[J].Fisheries Science & Technology Information,2002,29(4):163-165.(in Chinese)
ZHANG W,WANG Y J,LI W M,et al.Effects of transportation density and salinity on cortisol,glycogen and lactate of large yellow croaker (Larimichthys crocea) juveniles[J].Journal of Fisheries of China,2014,38(7):973-980.(in Chinese)
(11):59-60.(in Chinese)
YAN Y L,SHI Y H,ZHANG H M,et al.The anesthetic effect of MS-222 and eugenol on Coilia nasus Schlegel juvenile fish[J].Journal of Shanghai Ocean University,2016,25(2):177-182.(in Chinese)
QU H T,LIU Y,HU M H,et al. Anaesthetic effect of MS-222 on juveniles of Procypris rabaudi[J].Fisheries Science & Technology Information,2017,44(4):212-216.(in Chinese)
WANG W H,DONG H B,SUN Y X,et al.Anesthetic effects of MS-222 and eugenol in simulated transportation of juvenile largemouth bass (Micropterus salmoides)[J].South China Fisheries Science,2018,14(6):52-58.(in Chinese)
on Larimichthys crocea adult fish[J].Hunan Agricultural Sciences,2010(18):38-40.(in Chinese)
YANG L,ZHANG X L,LIU X D.Anesthetic effects of clove oil,MS-222 and propofol on Larimichthys crocea[J].Fishery Modernization,2021,48(2):91-96.(in Chinese)
LIU C L,HE L,CHEN S Q,et al.Review of fish anesthesia studies[J].Fishery Modernization,2007,34(5):21-25.(in Chinese)
LIN L Z,DU M X,YU L L,et al.Anesthetic effects of MS-222 on yellow catfish,Pelteobagrus fulvidraco[J].Fisheries Science & Technology Information,2019,46(1):56-60.(in Chinese)
,71. LI N,BAI C,XIONG G Q,et al.Comparision of metabolic residues of eugenol and tricaine methanesulfonate(MS-222) in large mouth bass[J].Modern Food Science and Technology,2020,36(3):56-64,71.(in Chinese)
CAO J,XIE J.Effect of 3-aminobenzoic acid ethyl ester methanesulfonate(MS-222) on improving the survival of turbot (Scophthalmus maximus) during simulated live transportation[J].Food Science,2021,42(23):246-253.(in Chinese)
HU F W,LI L,LIU Y W,et al.Anesthesia effect of clove oil and MS-222 on juvenile fat greenling Hexagrammos otakii[J].Journal of Dalian Ocean University,2017,32(6):671-675.(in Chinese)
on parrot fish[J].Modern Agricultural Science and Technology,2020(16):172-174.(in Chinese)
ZHAO M,LIU X Z,XU Y J,et al.Study on anaesthetic effects of MS-222 on adult Verasper variegates[J].Advances in Marine Science,2010,28(4):531-537.(in Chinese)
COOKE S J,SUSKI C D,OSTRAND K G,et al.Behavioral and physiological assessment of low concentrations of clove oil anaesthetic for handling and transporting largemouth bass (Micropterus salmoides)[J].Aquaculture,2004,239(1/2/3/4):509-529.
LI B Y,LU M,JIAO Y Q,et al.Progress on common fish anesthetics and their mechanism of action[J].Biology Teaching,2017,42(2):7-10.(in Chinese)
National Toxicology Program.NTP toxicology and carcinogenesis studies of methyleugenol (CAS No.93-15-2) in F344/N rats and B6C3F1 mice (gavage studies)[J].National Toxicology Program Technical Report Series,2000,491:1-412.
HILL J V,FORSTER M E.Cardiovascular responses of Chinook salmon (Oncorhynchus tshawytscha) during rapid anaesthetic induction and recovery[J].Comparative Biochemistry and Physiology Part C:Toxicology & Pharmacology,2004,137(2):167-177.
SUN W H,ZHAO D H,FU S L,et al.Anesthesia effects and accumulation and elimination regularity of MS-222 in turbot Scophthalmus maxinus[J].Journal of Food Safety & Quality,2015,6(11):4578-4583.(in Chinese)
LÜ X Y,LI J Y,FANG Y,et al.Nitrite stress on immune-related enzymes and the ultrastructure in different tissue of redclaw crayfish (Cherax quadricarinatus)[J].Journal of Fisheries of China,2010,34(12):1812-1820.(in Chinese)
ZHANG Z M,LIU D C,DING H P,et al.Effects of MS-222 and clove oil on blood biochemical parameters and immune indices of koi carp Cyprinus carpio[J].Fisheries Science,2015,34(2):71-76.(in Chinese)
WANG G Q,WANG W.Research progress in the effect of the stress response on fish[J].Journal of Anhui Agricultural Sciences,2009,37(24):11579-11580.(in Chinese)
NILSSON S,HOLMGREN S. Fish physiology:recent advances[M].Salt Lake City:Academic Press,1986.
(3):27-31.(in Chinese)
WANG L J,CHENG S K,ZHANG Y J,et al.Anesthetic effects of MS-222 in simulated transportation of largemouth bass(Micropterus salmoides)[J].Journal of Shanghai Ocean University,2015,24(2):235-241.(in Chinese)
DU H,WEI Q W,YANG D G,et al.Anaesthetic effects of MS-222,clove oil and benzocaine on cultured American shad Alosa sapidissima fingerlings[J].Journal of Dalian Fisheries University,2007,22(1):20-26.(in Chinese)
计量
- 文章访问数: 236
- PDF下载数: 0
- 施引文献: 0
下载:
