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ISSN 2095-1388

主管 辽宁省教育厅

主办 大连海洋大学

一株虾源假交替单胞菌(Pseudoalteromonas sp.)的分离鉴定及口服后对对虾体内弧菌的影响

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2023. 一株虾源假交替单胞菌(Pseudoalteromonas sp.)的分离鉴定及口服后对对虾体内弧菌的影响. 大连海洋大学学报, 38(2): 223-232. doi: 10.16535/j.cnki.dlhyxb.2022-150
引用本文: 2023. 一株虾源假交替单胞菌(Pseudoalteromonas sp.)的分离鉴定及口服后对对虾体内弧菌的影响. 大连海洋大学学报, 38(2): 223-232. doi: 10.16535/j.cnki.dlhyxb.2022-150
ZHU Na, WANG Xiuhua, ZHANG Hongfang, LI Ting, WANG Ping, ZHANG Xuemei. 2023. Isolation, identification and effects of a strain of Pseudoalteromonas sp. on Vibrio sp. in shrimp by oral administration. Journal of Dalian Ocean University, 38(2): 223-232. doi: 10.16535/j.cnki.dlhyxb.2022-150
Citation: ZHU Na, WANG Xiuhua, ZHANG Hongfang, LI Ting, WANG Ping, ZHANG Xuemei. 2023. Isolation, identification and effects of a strain of Pseudoalteromonas sp. on Vibrio sp. in shrimp by oral administration. Journal of Dalian Ocean University, 38(2): 223-232. doi: 10.16535/j.cnki.dlhyxb.2022-150

一株虾源假交替单胞菌(Pseudoalteromonas sp.)的分离鉴定及口服后对对虾体内弧菌的影响

  • 基金项目:

    国家重点研发计划"蓝色粮仓科技创新"项目(2019YFD0900105)

    "科技助力经济2020"重点专项(SQ2020YFF0425682)

    中国水产科学研究院黄海水产研究所基本科研业务费(20603022018001)

详细信息
    作者简介:

    朱娜(1996-),女,硕士研究生。E-mail:1364988922@qq.com

  • 中图分类号: S945.1

Isolation, identification and effects of a strain of Pseudoalteromonas sp. on Vibrio sp. in shrimp by oral administration

  • Fund Project: 中国水产科学研究院黄海水产研究所基本科研业务费(20603022018001)
  • 为探讨对虾细菌性病原的微生物防控技术,采用牛津杯打孔法对分离自对虾育苗池的一株编号为2021041402-14的细菌 (简称"02HN")进行了抗菌测试,分析其对欧文氏弧菌(Vibrio owensii)、哈维氏弧菌(V.harveyi)、坎贝氏弧菌(V.campbellii)、溶藻弧菌(V.alginolyticus)、副溶血弧菌(V.parahaemolyticus)和美人鱼发光杆菌(Photobacterium damselae) 等6株虾病原菌的抑菌效果及最低抑菌浓度,通过浸泡感染测试了菌株02HN对凡纳滨对虾(Litopenaeus vannamei)的生物毒性,采用16S rRNA、gyrB-rpoD串联基因序列比对法及生理生化方法对该菌进行了鉴定,并将该菌添加到饲料中投喂对虾,评价该菌对对虾体内弧菌数量的影响。结果表明:菌株02HN对欧文氏弧菌、哈维氏弧菌、坎贝氏弧菌、溶藻弧菌、副溶血弧菌和美人鱼发光杆菌均具有抑菌效果,其最低抑菌浓度分别为2.32×104、2.32×103、2.32×106、2.32×106、2.32×103、2.32×106 CFU/mL;采用02HN浓度为1×107 CFU/mL以下的菌液浸泡凡纳滨对虾,生物毒性不明显;分子鉴定显示,该菌与杀鱼假交替单胞菌(Pseudoalteromonas piscicida)及金丽假交替单胞菌(P.flavipulchra)最为接近,为一株假交替单胞菌(Pseudoalteromonas sp.);对虾摄食菌含量分别为1×105、1×107cells/g的饵料后,其存活及生长无显著性变化,但能显著降低对虾肝胰腺中的弧菌数量(P<0.05)。研究表明,菌株02HN在对虾养殖中具有开发应用潜能,本研究结果可为对虾细菌性病原的生物防控提供生物材料及技术思路。
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收稿日期:  2022-05-10

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