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ISSN 2095-1388

主管 辽宁省教育厅

主办 大连海洋大学

两种剂量下恩诺沙星及其代谢物环丙沙星在虹鳟体内的代谢残留

2021. 两种剂量下恩诺沙星及其代谢物环丙沙星在虹鳟体内的代谢残留. 大连海洋大学学报, 36(6): 985-994. doi: 10.16535/j.cnki.dlhyxb.2021-017
引用本文: 2021. 两种剂量下恩诺沙星及其代谢物环丙沙星在虹鳟体内的代谢残留. 大连海洋大学学报, 36(6): 985-994. doi: 10.16535/j.cnki.dlhyxb.2021-017
YU Runlin, CUI Shuyun, CHENG Bo, ZHANG Xin, KANG Tong, MU Yingchun, HAN Gang, SONG Yi. 2021. Pharmacokinetics of enrofloxacin and its metabolite ciprofloxacin in rainbow trout Oncorhynchus mykiss orally administrated at two doses. Journal of Dalian Ocean University, 36(6): 985-994. doi: 10.16535/j.cnki.dlhyxb.2021-017
Citation: YU Runlin, CUI Shuyun, CHENG Bo, ZHANG Xin, KANG Tong, MU Yingchun, HAN Gang, SONG Yi. 2021. Pharmacokinetics of enrofloxacin and its metabolite ciprofloxacin in rainbow trout Oncorhynchus mykiss orally administrated at two doses. Journal of Dalian Ocean University, 36(6): 985-994. doi: 10.16535/j.cnki.dlhyxb.2021-017

两种剂量下恩诺沙星及其代谢物环丙沙星在虹鳟体内的代谢残留

  • 基金项目:

    财政部和农业农村部:国家现代农业产业技术体系资助(CARS-46);中央级公益性科研院所基本科研业务费(2020B004)

详细信息
    作者简介:

    于润林(1995-),女,硕士研究生。E-mail:yurunliny@163.com

  • 中图分类号: S965.122

Pharmacokinetics of enrofloxacin and its metabolite ciprofloxacin in rainbow trout Oncorhynchus mykiss orally administrated at two doses

  • Fund Project: 财政部和农业农村部:国家现代农业产业技术体系资助(CARS-46);中央级公益性科研院所基本科研业务费(2020B004)
  • 为研究不同给药剂量下恩诺沙星(enrofloxacin,ENR)及其代谢物环丙沙星(ciprofloxacin,CIP)在虹鳟Oncorhynchus mykiss(体质量70.48 g±5.41 g)体内的代谢和消除规律,挑选健康虹鳟257尾,分为低剂量组(126尾,随机分为21个亚组,每组6尾)和高剂量组(132尾,随机分为22个亚组,每组6尾),在(15±2)℃条件下,分别以20、50 mg/kg生产实际使用剂量ENR对虹鳟进行单次口灌,于不同时间点采集其肝脏、肌肉、皮肤、肾脏、血浆和剩余组织,经高效液相色谱-串联质谱测定各组织药物浓度,采用DAS 2.0药代动力学软件用非房室模型统计矩方法分析药代参数并进行比较。结果表明:20 mg/kg剂量下,ENR在虹鳟肝脏、肌肉、皮肤、肾脏、剩余组织和血浆的药物达峰浓度(Cmax)值分别为13.349、11.164、23.378、114.196、33.762 mg/kg和3.361 mg/L,药物消除半衰期(t1/2z)分别为100.928、60.780、67.283、111.586、56.523、320.705 h;50 mg/kg剂量下,ENR在虹鳟对应组织的Cmax值分别为43.302、35.909、53.315、305.098、121.254 mg/kg和8.456 mg/L,t1/2z分别为204.193、115.639、102.405、121.852、201.834、325.593 h;两种剂量下虹鳟各组织均能检出环丙沙星,且高剂量下ENR及其代谢物CIP在虹鳟体内的吸收速度及程度均大于低剂量组,两种剂量下ENR和CIP均分别在虹鳟肾脏和肝脏中富集含量最高,高剂量下ENR消除速度较快,但残留时间较长。研究表明,在(15±2)℃下,建议ENR 20 mg/kg剂量对虹鳟单次口服给药休药期不低于768 h(32 d),50 mg/kg剂量下休药期不低于1 008 h(42 d)。
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收稿日期:  2021-01-22

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