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ISSN 2095-0780

CN 44-1683/S

主管 农业部

主办 中国水产科学研究院南海水产研究所

牡蛎肽对帕罗西汀致雄性小鼠性功能障碍的作用效果及潜在机制

2024. 牡蛎肽对帕罗西汀致雄性小鼠性功能障碍的作用效果及潜在机制. 南方水产科学, 20(1): 161-172. doi: 10.12131/20230153
引用本文: 2024. 牡蛎肽对帕罗西汀致雄性小鼠性功能障碍的作用效果及潜在机制. 南方水产科学, 20(1): 161-172. doi: 10.12131/20230153
Qianqian HUANG, Haiying WU, Xiaoming QIN. 2024. Effects and potential mechanism of oyster peptide on paroxetine-induced sexual dysfunction in male mice. South China Fisheries Science, 20(1): 161-172. doi: 10.12131/20230153
Citation: Qianqian HUANG, Haiying WU, Xiaoming QIN. 2024. Effects and potential mechanism of oyster peptide on paroxetine-induced sexual dysfunction in male mice. South China Fisheries Science, 20(1): 161-172. doi: 10.12131/20230153

牡蛎肽对帕罗西汀致雄性小鼠性功能障碍的作用效果及潜在机制

  • 基金项目:

    国家现代农业产业技术体系 (CARS-49)

详细信息
    作者简介:

    黄倩倩 (1998—),女,硕士研究生,研究方向为贝类活性物质。E-mail: 953025724@qq.com

    通讯作者: 秦小明 (1964—),男,教授,博士,研究方向为贝类产品加工以及水产品保活运输等。E-mail: qinxm@gdou.edu.cn
  • 中图分类号: TS 254.1

Effects and potential mechanism of oyster peptide on paroxetine-induced sexual dysfunction in male mice

  • Fund Project: 国家现代农业产业技术体系 (CARS-49)
More Information
  • 牡蛎肽 (Oyster peptide, OP) 具有多种生物活性,然而,其对男性性功能障碍的作用效果仍知之甚少。以牡蛎肽为研究对象,探讨其对男性性功能障碍的作用效果及其潜在机制。每天灌胃帕罗西汀 (Paroxetine, PRX) 构建雄性小鼠性功能障碍模型,同时灌胃牡蛎肽 (500 mg·kg−1),持续28 d。结果表明:与模型 (PRX) 组小鼠相比,牡蛎肽可显著提高雄性小鼠的性能力 (P<0.05),恢复血清性激素水平 (P<0.01),提高阴茎组织一氧化氮 (NO) 含量 (P<0.01)、环磷酸鸟苷 (cGMP) 含量 (P<0.05), 和一氧化氮合酶 (NOS) 活性 (P<0.05),并降低磷酸二酯酶-5 (PDE-5) 活性 (P<0.01);同时,牡蛎肽可增强睾丸标志性酶活性 (P<0.05) 和抗氧化能力 (P<0.01),改善精子质量。此外,HE染色结果显示:牡蛎肽可恢复小鼠睾丸生精小管内生精细胞的数量与形态,减少生精小管空泡化现象。综上所述,牡蛎肽可有效减缓PRX导致的雄性小鼠性功能障碍,推测其对男性性功能障碍具有潜在的保护作用。
  • 加载中
  • 图 1  牡蛎肽相对分子质量分布色谱图

    Figure 1.  Chromatogram of relative molecular mass distribution of oyster peptides

    图 2  牡蛎肽对小鼠交配行为的影响

    Figure 2.  Effect of oyster peptid on sexual behavior in mice

    图 3  牡蛎肽对血清性激素的影响

    Figure 3.  Effect of oyster peptid on serum sex hormones

    图 4  牡蛎肽对阴茎组织一氧化氮、一氧化氮合酶、环磷酸鸟苷、磷酸二酯酶-5含量或活性的影响

    Figure 4.  Effect of OP on content or activity of NO, NOS, cGMP and PDE5 in penile tissue

    图 5  牡蛎肽对睾丸组织标志性酶的影响

    Figure 5.  Effect of oyster peptid on marker enzymes of testis tissue

    图 6  牡蛎肽对睾丸组织抗氧化能力的影响

    Figure 6.  Effect of oyster peptid on antioxidant capacity of testis tissue

    图 7  睾丸组织病理分析

    Figure 7.  Pathological analysis of testicular tissue

    图 8  精子数量与活率

    Figure 8.  Sperm count and motility

    图 9  精子形态分析

    Figure 9.  Sperm morphology analysis

    表 1  牡蛎肽游离氨基酸含量分析

    Table 1.  Analysis of free amino acid content of oyster peptid

    序号
    No.
    游离氨基酸
    Free amino acid
    质量分数或含量
    Mass fraction or content/%
    1天门冬氨酸 Asp0.39±0.032
    2苏氨酸 Thr0.41±0.031
    3丝氨酸 Ser0.30±0.021
    4谷氨酸 Glu0.86±0.052
    5脯氨酸 Pro0.07±0.015
    6甘氨酸 Pro0.31±0.033
    7丙氨酸 Ala1.26±0.065
    8胱氨酸 Cys0.04±0.000
    9缬氨酸 Val①②③0.57±0.145
    10蛋氨酸 Met②③0.26±0.036
    11异亮氨酸 Ile①②③0.76±0.111
    12亮氨酸 Leu①②③1.68±0.166
    13酪氨酸 Tyr0.96±0.030
    14苯丙氨酸 Phe①②1.13±0.267
    15赖氨酸 Lys2.14±0.248
    16组氨酸 His0.29±0.019
    17精氨酸 Arg2.68±0.142
    氨基酸总量
    Total amino acid, TAA
    14.10
    疏水性氨基酸
    Hydrophobic amino acid, HAA
    5.74
    必需氨基酸
    Essential amino acid, EAA
    6.95
    支链氨基酸
    Branched-chain amino acids, BCAA
    3.01
    EAA/TAA (%)49.29
    BCAA/TAA (%)21.35
    HAA/TAA (%)40.71
    注:n=3;① 必需氨基酸;② 疏水性氨基酸;③ 支链氨基酸。 Note: n=3; ① Essential amino acid; ② Hydrophobic amino acid; ③ A branched-chain amino acid.
    下载: 导出CSV

    表 2  牡蛎肽对脏器系数的影响

    Table 2.  Effect of oyster peptid on organ coefficient %

    项目
    Item
    空白组
    CN
    模型组
    PRX
    阳性组
    PRX+SDF
    牡蛎肽组
    PRX+OP
    心脏 Heart 0.599±0.067 0.581±0.072 0.597±0.082 0.622±0.079
    胸腺 Thymus 0.11±0.029 0.104±0.021 0.116±0.46 0.113±0.022
    脾 Lien 0.328±0.023 0.305±0.026 0.335±0.042 0.309±0.041
    肝脏 Liver 5.106±0.586 4.798±0.351 5.004±0.581 5.018±0.306
    肾脏 Ren 1.682±0.117 1.629±0.094 1.638±0.151 1.669±0.069
    肺 Lung 0.657±0.061 0.637±0.048 0.641±0.034 0.652±0.039
    阴茎 Penis 0.120±0.019 0.109±0.026 0.119±0.017 0.119±0.012
    睾丸 Testis 0.759±0.083 0.629±0.061*** 0.699±0.069# 0.685±0.037#
    精囊腺 Seminal vesicle 0.839±0.108 0.574±0.097*** 0.687±0.084# 0.731±0.061###
    下载: 导出CSV
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出版历程
收稿日期:  2023-08-08
修回日期:  2023-09-14
录用日期:  2023-10-18
刊出日期:  2024-02-05

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