Effects and potential mechanism of oyster peptide on paroxetine-induced sexual dysfunction in male mice
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摘要: 牡蛎肽 (Oyster peptide, OP) 具有多种生物活性，然而，其对男性性功能障碍的作用效果仍知之甚少。以牡蛎肽为研究对象，探讨其对男性性功能障碍的作用效果及其潜在机制。每天灌胃帕罗西汀 (Paroxetine, PRX) 构建雄性小鼠性功能障碍模型，同时灌胃牡蛎肽 (500 mg·kg−1)，持续28 d。结果表明：与模型 (PRX) 组小鼠相比，牡蛎肽可显著提高雄性小鼠的性能力 (P<0.05)，恢复血清性激素水平 (P<0.01)，提高阴茎组织一氧化氮 (NO) 含量 (P<0.01)、环磷酸鸟苷 (cGMP) 含量 (P<0.05), 和一氧化氮合酶 (NOS) 活性 (P<0.05)，并降低磷酸二酯酶-5 (PDE-5) 活性 (P<0.01)；同时，牡蛎肽可增强睾丸标志性酶活性 (P<0.05) 和抗氧化能力 (P<0.01)，改善精子质量。此外，HE染色结果显示：牡蛎肽可恢复小鼠睾丸生精小管内生精细胞的数量与形态，减少生精小管空泡化现象。综上所述，牡蛎肽可有效减缓PRX导致的雄性小鼠性功能障碍，推测其对男性性功能障碍具有潜在的保护作用。Abstract: Oyster peptide (OP) has various biological activities. However, its effects on male sexual dysfunction is still poorly understood. In this study, we explored its effects and potential mechanism on male sexual dysfunction. Besides, we established a paroxetine (PRX)-induced sexual dysfunction model by gavaging OP (500 mg·kg− 1) in mice for 28 d. The results show that compared with the model (PRX) group, OP could improve the sexual performance of male mice (P<0.05), restored serum sex hormone levels (P<0.01), increased penile tissue nitric oxide (NO) content (P<0.01), cyclic guanosine monophosphate (cGMP) content (P<0.05) and nitric oxide synthase (NOS) activity (P<0.05), and decreased phosphodiesterase-5 (PDE-5) activity (P<0.01). Meanwhile, OP enhanced testicular marker enzymes activities (P<0.05) and antioxidant capacity (P<0.01), and improved sperm quality. In addition, HE staining results show that OP could restore the number and morphology of spermatogenic cells in seminiferous tubules of mice, and reduced the vacuolization of seminiferous tubules. In conclusion, OP can alleviate PRX-induced sexual dysfunction effectively in male mice and has a potential protective effect on male sexual dysfunction.
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Key words:
- Oyster peptide /
- Paroxetine /
- Sexual dysfunction /
- Erectile dysfunction /
- Sexual behavior
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图 1 牡蛎肽相对分子质量分布色谱图
Figure 1. Chromatogram of relative molecular mass distribution of oyster peptides
图 4 牡蛎肽对阴茎组织一氧化氮、一氧化氮合酶、环磷酸鸟苷、磷酸二酯酶-5含量或活性的影响
Figure 4. Effect of OP on content or activity of NO, NOS, cGMP and PDE5 in penile tissue
图 5 牡蛎肽对睾丸组织标志性酶的影响
Figure 5. Effect of oyster peptid on marker enzymes of testis tissue
图 6 牡蛎肽对睾丸组织抗氧化能力的影响
Figure 6. Effect of oyster peptid on antioxidant capacity of testis tissue
表 1 牡蛎肽游离氨基酸含量分析
Table 1. Analysis of free amino acid content of oyster peptid
序号
No.游离氨基酸
Free amino acid质量分数或含量
Mass fraction or content/%1 天门冬氨酸 Asp 0.39±0.032 2 苏氨酸 Thr① 0.41±0.031 3 丝氨酸 Ser 0.30±0.021 4 谷氨酸 Glu 0.86±0.052 5 脯氨酸 Pro② 0.07±0.015 6 甘氨酸 Pro 0.31±0.033 7 丙氨酸 Ala② 1.26±0.065 8 胱氨酸 Cys 0.04±0.000 9 缬氨酸 Val①②③ 0.57±0.145 10 蛋氨酸 Met②③ 0.26±0.036 11 异亮氨酸 Ile①②③ 0.76±0.111 12 亮氨酸 Leu①②③ 1.68±0.166 13 酪氨酸 Tyr 0.96±0.030 14 苯丙氨酸 Phe①② 1.13±0.267 15 赖氨酸 Lys① 2.14±0.248 16 组氨酸 His 0.29±0.019 17 精氨酸 Arg 2.68±0.142 氨基酸总量
Total amino acid, TAA14.10 疏水性氨基酸
Hydrophobic amino acid, HAA5.74 必需氨基酸
Essential amino acid, EAA6.95 支链氨基酸
Branched-chain amino acids, BCAA3.01 EAA/TAA (%) 49.29 BCAA/TAA (%) 21.35 HAA/TAA (%) 40.71 注：n=3；① 必需氨基酸；② 疏水性氨基酸；③ 支链氨基酸。 Note: n=3; ① Essential amino acid; ② Hydrophobic amino acid; ③ A branched-chain amino acid. 
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表 2 牡蛎肽对脏器系数的影响
Table 2. Effect of oyster peptid on organ coefficient
% 项目
Item空白组
CN模型组
PRX阳性组
PRX+SDF牡蛎肽组
PRX+OP心脏 Heart 0.599±0.067 0.581±0.072 0.597±0.082 0.622±0.079 胸腺 Thymus 0.11±0.029 0.104±0.021 0.116±0.46 0.113±0.022 脾 Lien 0.328±0.023 0.305±0.026 0.335±0.042 0.309±0.041 肝脏 Liver 5.106±0.586 4.798±0.351 5.004±0.581 5.018±0.306 肾脏 Ren 1.682±0.117 1.629±0.094 1.638±0.151 1.669±0.069 肺 Lung 0.657±0.061 0.637±0.048 0.641±0.034 0.652±0.039 阴茎 Penis 0.120±0.019 0.109±0.026 0.119±0.017 0.119±0.012 睾丸 Testis 0.759±0.083 0.629±0.061*** 0.699±0.069# 0.685±0.037# 精囊腺 Seminal vesicle 0.839±0.108 0.574±0.097*** 0.687±0.084# 0.731±0.061###
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