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ISSN 2095-0780

CN 44-1683/S

主管 农业部

主办 中国水产科学研究院南海水产研究所

珍珠贝外套膜酶解产物促进皮肤创伤愈合效果研究

2019. 珍珠贝外套膜酶解产物促进皮肤创伤愈合效果研究. 南方水产科学, 15(5): 92-98. doi: 10.12131/20190079
引用本文: 2019. 珍珠贝外套膜酶解产物促进皮肤创伤愈合效果研究. 南方水产科学, 15(5): 92-98. doi: 10.12131/20190079
Faming YANG, Haisheng LIN, Xiaoming QIN, Chaohua ZHANG, Wenhong CAO, Jialong GAO. 2019. Effect of enzymatic hydrolysis from mantle of pearl oyster on skin wound healing. South China Fisheries Science, 15(5): 92-98. doi: 10.12131/20190079
Citation: Faming YANG, Haisheng LIN, Xiaoming QIN, Chaohua ZHANG, Wenhong CAO, Jialong GAO. 2019. Effect of enzymatic hydrolysis from mantle of pearl oyster on skin wound healing. South China Fisheries Science, 15(5): 92-98. doi: 10.12131/20190079

珍珠贝外套膜酶解产物促进皮肤创伤愈合效果研究

  • 基金项目:

    现代农业产业技术体系建设专项资金 (CARS-49);广东海洋大学博士启动项目 (R17082);广东海洋大学“海之帆”起航计划大学生科技创新培育项目 (230419038);广东普通高等学校水产品高值化加工与利用创新团队项目 (GDOU2016030503);广东省应用型科技研发专项资金项目 (2016B020235002)

详细信息
    作者简介:

    杨发明 (1995—),男,硕士研究生,从事海洋功能活性物质研究。Email: yangfm0123@163.com

    通讯作者: 秦小明 (1964—),男,博士,教授,从事水产品精深加工、贝类活性物质研究。E-mail: xiaoming0502@21cn.com
  • 中图分类号: TS 254.9

Effect of enzymatic hydrolysis from mantle of pearl oyster on skin wound healing

  • Fund Project: 现代农业产业技术体系建设专项资金 (CARS-49);广东海洋大学博士启动项目 (R17082);广东海洋大学“海之帆”起航计划大学生科技创新培育项目 (230419038);广东普通高等学校水产品高值化加工与利用创新团队项目 (GDOU2016030503);广东省应用型科技研发专项资金项目 (2016B020235002)
More Information
  • 为进一步拓宽马氏珠母贝(Pinctada martensii)的高值化利用途径,文章探讨了马氏珠母贝外套膜酶解产物体外抗菌活性、促凝血作用以及对小鼠软组织开放性创伤愈合效果。结果表明,珍珠贝外套膜酶解产物具有一定的促凝血作用,但对13种测试菌无抑制作用;涂抹给药6 d后,给药组小鼠的创伤愈合率显著高于阴性对照组(P<0.05),与阳性对照组无显著差异(P>0.05);给药组的第14天愈合率达100%,疤痕缩小率显著高于阴性对照组(P<0.05)。生化指标测试结果显示,与2个对照组相比,马氏珠母贝外套膜酶解产物能够抑制炎症因子IL-6生成;与阴性对照组相比,其可极显著促进IL-10分泌;与阴性对照组相比,给药组皮肤组织中FGF-2含量无显著性差异(P>0.05),阳性对照组EGF含量显著高于其他组(P<0.05);而TGF-β和CCND1含量均显著高于阴性对照组(P<0.05)。综上,马氏珠母贝外套膜酶解产物具有一定促凝血、抑制炎症作用并促进生长因子分泌,从而加快小鼠软组织开放性创伤愈合,对浅表瘢痕增生具有一定的抑制作用。
  • 加载中
  • 图 1  给药组对小鼠创面的影响

    Figure 1.  Effect of drug group on wound of mice

    图 2  第14天马氏珠母贝外套膜酶解产物对小鼠皮肤创伤瘢痕生成的影响

    Figure 2.  Effect of EHM on scar formation of skin wounds of mice on Day 14

    图 3  马氏珠母贝外套膜酶解产物对创伤皮肤组织中炎症因子的影响

    Figure 3.  Effect of EHM on inflammatory factors in wound skin tissue

    表 1  马氏珠母贝外套膜酶解产物对小鼠断尾创口的出血时间和出血量的影响

    Table 1.  Effect of EHM on bleeding time and blood loss of tail            wound of mice        $\overline {\boldsymbol X} {\bf \pm} {\bf{SD}}$, n=5

    组别group出血时间/minbleeding time出血量/gamount of bleeding
    阴性对照组 negative control 5.05±0.79c 0.015 2±0.001 9c
    阳性对照组 positive control 0.94±0.49b 0.005 4±0.001 7b
    给药组 drug group 1.97±0.26a 0.009 6±0.001 8a
    注:同列中标有不同小写字母者表示组间有显著性差异 (P<0.05),标有相同小写字母者表示组间无显著性差异 (P>0.05),下表、后图同此 Note: Different and same letters within the same row indicate significant (P<0.05) and insignificant different (P>0.05), respectively. The same case in the following tables and figures.
    下载: 导出CSV

    表 2  马氏珠母贝外套膜酶解产物对小鼠皮肤创伤愈合的影响

    Table 2.  Effect of EHM on skin wound healing of mice $\overline {\boldsymbol X} {\bf \pm} {\bf{SD;}}\;$ %

    组别group第0天Day 0第6天Day 6第10天Day 10第14天Day 14
    阴性对照组 negative control 0±0.00 14.17±2.89b 35.86±8.66b 80.42±6.17b
    阳性对照组 positive control 0±0.00 35.83±6.88a 56.67±17.02a 92.92±6.41a
    给药组 drug group 0±0.00 28.33±6.88a 87.50±11.11a 100.00±0.00a
    下载: 导出CSV

    表 3  马氏珠母贝外套膜酶解产物对创伤皮肤组织中生长因子的影响

    Table 3.  Effect of EHM on growth factors in wound skin tissue on Day 7 $\overline {\boldsymbol X} {\bf \pm} {\bf{SD}}$; μg·mL–1

    组别group成纤维细胞生长因子2FGF-2转化生长因子βTGF-β细胞生长周期素1CCND1表皮生长因子EGF
    阴性对照组 negative control 36.58±2.65a 0.44±0.04b 1.67±0.50a 131.37±59.51a
    阳性对照组 positive control 28.72±5.65a 1.55±0.11a 2.74±1.21a 1 674.34±913.78b
    给药组 drug group 32.60±5.00a 2.09±0.89a 6.60±1.63b 660.59±376.41a
    下载: 导出CSV

    表 4  马氏珠母贝外套膜酶解产物对创伤皮肤组织中羟脯氨酸的影响

    Table 4.  Effect of EHM on hydroxyproline in wound skin tissue              $\overline {\boldsymbol X} {\bf \pm} {\bf{SD}} $; μg·mL–1

    组别group造模后时间/d post-moulding time
    714
    阴性对照组 negative control 7.58±0.00ab 13.10±2.11b
    阳性对照组 positive control 4.60±0.99a 23.86±2.73b
    给药组 drug group 4.10±0.89ac 10.28±1.12a
    下载: 导出CSV
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出版历程
收稿日期:  2019-04-05
修回日期:  2019-06-10
刊出日期:  2019-10-05

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