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ISSN 2095-0780

CN 44-1683/S

主管 农业部

主办 中国水产科学研究院南海水产研究所

锦鲤早期发育过程中色素代谢相关酶的活性和基因表达变化

2019. 锦鲤早期发育过程中色素代谢相关酶的活性和基因表达变化. 南方水产科学, 15(5): 109-117. doi: 10.12131/20190023
引用本文: 2019. 锦鲤早期发育过程中色素代谢相关酶的活性和基因表达变化. 南方水产科学, 15(5): 109-117. doi: 10.12131/20190023
Haoran YIN, Mingkun LUO, Lanmei WANG, Zaijie DONG, Wenbin ZHU, Jianjun FU. 2019. Changes of pigment-related enzyme activity and gene expression at early developmental stage of koi carp. South China Fisheries Science, 15(5): 109-117. doi: 10.12131/20190023
Citation: Haoran YIN, Mingkun LUO, Lanmei WANG, Zaijie DONG, Wenbin ZHU, Jianjun FU. 2019. Changes of pigment-related enzyme activity and gene expression at early developmental stage of koi carp. South China Fisheries Science, 15(5): 109-117. doi: 10.12131/20190023

锦鲤早期发育过程中色素代谢相关酶的活性和基因表达变化

  • 基金项目:

    江苏省第五期“333工程”培养资金 (BRA2017083);农业农村部现代农业人才支撑计划项目 (2016142);中央级公益性科研院所基本科研业务费专项资金(2019ZY22)

详细信息
    作者简介:

    殷浩然(1995— ),男,硕士研究生,从事水产动物遗传育种研究。E-mail: 1334733261@qq.com

    通讯作者: 董在杰(1967— ),男,博士,研究员,从事水生动物遗传育种研究。E-mail: dongzj@ffrc.cn
  • 中图分类号: S 965.8

Changes of pigment-related enzyme activity and gene expression at early developmental stage of koi carp

  • Fund Project: 江苏省第五期“333工程”培养资金 (BRA2017083);农业农村部现代农业人才支撑计划项目 (2016142);中央级公益性科研院所基本科研业务费专项资金(2019ZY22)
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  • 通过显微观察、酶联免疫吸附及荧光定量PCR等方法,探究了红白、红黑锦鲤(Cyprinus carpio var. koi) 子一代 (F1)早期体色发育过程及不同发育时期色素代谢相关酶(酪氨酸酶、胱氨酸酶)的活性和基因(agoutimc1rmitftyr)的表达变化。结果表明,锦鲤出膜后呈淡黄色半透明,黑色素细胞在出膜8 d后开始出现。胱氨酸酶活性随锦鲤发育呈升高趋势,出膜7 d后显著升高(P<0.05);7日龄仔鱼前,红黑组与红白组间无显著差异(P>0.05);出膜7 d后红黑组比同时期红白组显著升高(P<0.05)。酪氨酸酶在锦鲤发育过程中也呈升高趋势,但略有起伏,眼色素积累期及出膜23 d后酪氨酸酶活性均比相邻各期高(P<0.05);组间比较发现发育各期红黑组均比红白组高(P<0.05)。表明酪氨酸酶、胱氨酸酶活性变化与锦鲤色素细胞发育存在密切关联。agoutimc1rmitftyr 4个基因均在原肠期与神经胚期时表达最高(P<0.01),随后均呈下降趋势,表明这4个基因可能在胚胎发育早期的体色形成过程中发挥着重要作用。
  • 加载中
  • 图 1  锦鲤早期发育体色变化

    Figure 1.  Change of body color at early developmental stage of koi carp

    图 2  红黑组与红白组发育过程中胱氨酸酶活性的变化

    Figure 2.  Change of cystinase activity during embryonic development of Kohaku group and Hi-Utsuri group

    图 3  红黑组与红白组发育过程中酪氨酸酶活性的变化

    Figure 3.  Chang of tyrosinase activity during embryonic development of Kohaku group and Hi-Utsuri group

    图 4  agouti基因在锦鲤不同发育时期表达量

    Figure 4.  agouti gene expression at different developmental stage

    图 5  mc1r基因在锦鲤不同发育时期表达量

    Figure 5.  mc1r gene expression at different developmental stage

    图 6  mitf基因在锦鲤不同发育时期表达量

    Figure 6.  mitf gene expression at different developmental stage

    图 7  tyr基因在锦鲤不同发育时期表达量

    Figure 7.  tyr gene expression at different developmental stage

    表 1  实时荧光定量PCR引物

    Table 1.  Primer sequences used in real-time quantitative RT-PCR

    引物名称primer核苷酸序列 (5'−3')sequence
    tyr FCACGGTCTCCGATCTTCCC
    tyr RCATCACGCCAGTCCCAGTA
    mitf FACAACTCCTGCCCGTCTAAC
    mitf RCCGTTGTTGAGGTCCAGAGT
    agouti FAACTGCGTGCCGCTCTTGA
    agouti RTAAACAAGCCTTTGGGATCGGG
    mc1r FAGGAATCTCCACTCGCCAA
    mc1r RGCCCGTGCTCCGTCAATAA
    β-actin FAGTCAGCAGAGTTGGGTGCT
    β-actin RCACGGCATCATTACCAACTG
    下载: 导出CSV

    表 2  红白组子一代体色性状分离

    Table 2.  Segregation of body color characteristics of F1 generation in Kohaku group

    组别 group全红 red全白 white红白 Kohaku
    红白A Kohaku A个体数463200441
    百分比/%41.9418.139.95
    红白B Kohaku B个体数377180652
    百分比/%31.1814.8953.93
    下载: 导出CSV

    表 3  红黑组子一代体色性状分离

    Table 3.  Segregation of body color characteristics of F1 generation in Hi-Utsuri group

    组别 group全红 red红黑 Hi-Utsuri全白 white红白 Kohaku三色 Showa黑白 Shiro-Utsuri
    红黑A Hi-Utsuri A个体数31249829723313
    百分比/%27.0143.120.178.4020.171.13
    红黑B Hi-Utsuri B个体数9613466218403216
    百分比/%8.4711.835.8319.2435.5719.06
    下载: 导出CSV

    表 4  红黑组子一代黑色斑纹分离

    Table 4.  Segregation of black pattern characteristics of F1 generation in Hi-Utsuri group

     组别 group有黑斑 pigmented无黑斑 unpigmented
    红黑A Hi-Utsuri A个体数744411
    比例1.81∶1
    红黑B Hi-Utsuri B个体数753380
    比例1.98∶1
    下载: 导出CSV
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出版历程
收稿日期:  2019-01-28
修回日期:  2019-05-16
刊出日期:  2019-10-05

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