Effects of salinity on survival, growth and antioxidant defense system of Scatophagus argus
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摘要: 为探讨盐度对金钱鱼（Scatophagus argus）幼鱼存活、生长和抗氧化状态的影响，在6个盐度梯度（淡水和盐度5、10、20、30和35）下，养殖体质量为（6.27±0.28） g金钱鱼幼鱼60 d，统计其存活率及生长情况，测定各盐度下金钱鱼幼鱼肝脏、鳃、肌肉组织中超氧化物歧化酶（SOD）、过氧化氢酶（CAT）、谷胱甘肽过氧化物酶（GPx）、谷胱甘肽S-转移酶（GST）活力，以及还原型谷胱甘肽（GSH）和丙二醛（MDA）含量。结果表明：金钱鱼幼鱼在盐度为0~35时存活率为97.78%~100%，无显著性差异（P>0.05）。盐度为5时，终末体长、体质量和增重率均达到最大，盐度为35时，终末体长、体质量和增重率最小。盐度5条件下的特定生长率为（0.96±0.01）%，显著高于其他盐度组（P<0.05）。基于特定生长率的二次回归模型拟合，预测体质量为（6.27±0.28） g的金钱鱼幼鱼高效养殖盐度为7.5。淡水组和盐度35高盐组金钱鱼幼鱼肝脏中SOD与CAT活力显著高于其他各组（P<0.05），并且肝脏中的GPx活力和MDA含量也处于较高水平。GST活力与GSH含量在各盐度组间没有显著性差异（P>0.05）。研究揭示：金钱鱼幼鱼在0~35的盐度时具有高存活率，但在低盐环境中生长较快；淡水和高盐环境能引起鱼体内产生一定程度的抗氧化应激。推荐金钱鱼幼鱼养殖盐度为5~10。Abstract: In order to study the effects of salinity on the survival, growth and antioxidant status of juveniles Scatophagus argus, fish with average body weight of (6.27±0.28) g were exposed to different salinities groups (0, 5, 10, 20, 30, 35) and reared for 60 days, and the survival rate and growth status were counted. The superoxide dismutase (SOD), catalase (CAT), glutathione peroxidase (GPx), and glutathione S-transferase (GST) activities, glutathione (GSH) and malondialdehyde (MDA) contents in liver, gill and muscle tissues were determined. The results showed that:The survival rate was 97.78%~100%, and there was no significant difference among the different salinities groups (P>0.05). When the salinity was 5, the final body length, body weight and weight gain rate showed the maximum, while these values showed the minimum when the salinity was 35. The specific growth rate (SGR) was (0.96±0.01)% at the salinity of 5, which was significantly higher than other groups (P<0.05). The optimal breeding salinity of juvenile Scatophagus argus with body mass of (6.27±0.28) g was 7.5, obtained by the quadratic regression model fitting based on the SGR. The SOD and CAT activities in the liver of fresh water group and salinity 35 group were significantly higher than those in other groups (P<0.05), the GPx activity and MDA content in liver were also at a higher level. There were no significant differences in the activity of GST and GSH content among different salinities (P>0.05). The results showed that:The survival rate of Scatophagus argus juveniles was high in the salinity range of 0-35, but juvenile Scatophagus argus grew faster in the low permeability environment; The fresh water and hypertonic environment caused a certain degree of oxidative stress in fish. It is recommended to raise young juvenile Scatophagus argus in the salinity range of 5-10.
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Key words:
- Scatophagus argus /
- salinity /
- survival /
- growth /
- antioxidant enzymes
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