Effects of “reverse back syndrome” on main digestive enzymes and immune-related enzymes in Babylonia areolata
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摘要: 为探索方斑东风螺消化及免疫系统对“翻背症”的应答机制，采集了健康(对照组)和“翻背症”早期、中期和晚期的方斑东风螺，采用TCBS平板计数法检测螺体内弧菌含量，试剂盒法检测螺体内组织超氧化物歧化酶(SOD)、过氧化物酶(POD)、过氧化氢酶(CAT)、酸性磷酸酶(ACP)、碱性磷酸酶(AKP)、溶菌酶(LZM)等免疫相关酶和胃蛋白酶、脂肪酶、淀粉酶等消化酶活性。结果显示，方斑东风螺体内弧菌含量随病情的发展而显著增加，晚期达到最大值。发病早期，CAT、SOD、POD、ACP和胃蛋白酶等酶活性均显著上升。发病中期，POD、AKP、LZM活性均上升，且显著高于对照组和发病早期；与发病早期相比，CAT、SOD、ACP、胃蛋白酶、脂肪酶活性有所下降；与对照组相比，CAT、ACP活性仍显著升高，胃蛋白酶、脂肪酶活性显著降低，而SOD、淀粉酶活性无显著变化。发病晚期，各消化酶与免疫相关酶活性均较发病中期有所下降，但POD、ACP活性仍显著高于对照组，CAT、SOD、AKP、LZM活性与对照组无显著差异，而胃蛋白酶、脂肪酶、淀粉酶活性显著低于对照组。研究表明，“翻背症”方斑东风螺体内弧菌的暴发过程中，机体的免疫系统与消化系统均参与了机体病害免疫应答反应，且可以采用CAT、POD和ACP活性作为方斑东风螺“翻背症”的检测评价指标。Abstract: To explore the response mechanism of digestion and immune system to “reverse back syndrome” (RBS) of Babylonia areolata, both healthy(control group, CG) and disease samples (early stage of RBS [ERBS], middle stage of RBS [MRBS], and late stage of RBS [ERBS]) were collected, the Vibrio content, the activity of SOD, POD, CAT, ACP, AKP, LZM, pepsin, lipase and amylase in these samples were detected by TCBS plate count methods and enzyme test kit methods. The results showed that the content of Vibrio increased significantly with the development of the disease, and it reached the maximum in LRBS of Babylonia areolata. In MRBS, the activity of POD, AKP and LZM increased, and significantly higher than CG and ERBS; compared with ERBS, the activity of CAT, SOD, ACP, pepsin and lipase decreased; but compared with CG, the CAT and ACP activity increased significantly, and the pepsin and lipase activity decreased significantly, while there was no significant difference in SOD and amylase activity. In LRBS, the activities of digestive enzymes and immune-related enzymes were lower than those in MRBS, and compared with CG, the POD and ACP activity significantly increased, the pepsin, lipase and amylase activity significantly decreased, while there was no significant difference in the CAT, SOD, AKP and LZM activity. In conclusion, the immune system and digestive system are involved in the immune response during the outbreak of Vibrio in the RBS, and the CAT, POD and ACP activity can be used as the detection and evaluation indicators.
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Key words:
- Babylonia areolata /
- reverse back syndrome /
- digestive enzyme /
- immune enzyme
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图 2 “翻背症”对方斑东风螺抗氧化酶活性的影响
Figure 2. Effect of RBS on the anti-oxidase activity of B. areolata
图 3 “翻背症”对方斑东风螺磷酸酶、溶菌酶活性的影响
Figure 3. Effect of RBS on the phosphatase and LZM activity of B. areolata
图 4 “翻背症”对方斑东风螺消化酶活性的影响
Figure 4. Effect of RBS on the digestive enzyme activity of B. areolata
表 1 “翻背症”方斑东风螺消化及免疫相关酶活性对弧菌含量的回归分析
Table 1. Regression analysis of digestion and immune-related enzyme activity on Vibrio content of RBS of B. areolata
酶
enzymeR2 F P 回归方程系数 coefficient of regression equation b0 b1 b2 b3 CAT 0.835 22.721 0 −1 713.081 444.289 0 −3.800 SOD 0.442 3.564 0.072 −412.673 124.610 0 −1.040 POD 0.780 15.956 0.001 −7 111.196 1 744.026 0 −14.377 ACP 0.767 14.836 0.001 −13 474.571 4 366.968 −345.580 0 AKP 0.390 2.875 0.108 −4 489.302 1 121.022 0 −9.520 LZM 0.438 3.502 0.075 −4 685.078 1 176.620 0 −10.184 胃蛋白酶 pepsin 0.489 4.312 0.049 −2 196.170 578.656 0 −5.330 脂肪酶 lipase 0.622 7.411 0.013 −1 148.332 318.686 0 −3.033 淀粉酶 amylase 0.440 3.531 0.074 −148.224 65.229 0 −0.663 
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